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Anti-Pumilio 2 antibody [EPR3813] (ab92390)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3813] to Pumilio 2
  • Suitable for: Flow Cyt, WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Pumilio 2 antibody [EPR3813]
    See all Pumilio 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3813] to Pumilio 2

  • Host species

    Rabbit

  • Specificity

    This antibody is predicted to not cross react with Pumilio 1.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Pumilio 2 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa and 293T cell lysates ICC/IF: MCF-7 cells. Flow Cyt: HeLa cells IP: HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Pumilio 2 antibody [EPR3813] (ab92390)
    Western blot - Anti-Pumilio 2 antibody [EPR3813] (ab92390)
    All lanes : Anti-Pumilio 2 antibody [EPR3813] (ab92390) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 114 kDa
    Observed band size: 114 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Pumilio 2 antibody [EPR3813] (ab92390)
    Immunocytochemistry/ Immunofluorescence - Anti-Pumilio 2 antibody [EPR3813] (ab92390)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Pumilio 2 with purified ab92390 at 1/50 dilution (4.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-Pumilio 2 antibody [EPR3813] (ab92390)
    Flow Cytometry - Anti-Pumilio 2 antibody [EPR3813] (ab92390)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Pumilio 2 with purified ab92390 at 1/30 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-Pumilio 2 antibody [EPR3813] (ab92390)
    Immunoprecipitation - Anti-Pumilio 2 antibody [EPR3813] (ab92390)

    ab92390 (purified) at 1/20 dilution (1ug) immunoprecipitating Pumilio 2 in HeLa whole cell lysates.
    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug
    Lane 2 (+): ab92390 & HeLa whole cell lysates
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92390 in HeLa whole cell lysates
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-Pumilio 2 antibody [EPR3813] (ab92390)
    Western blot - Anti-Pumilio 2 antibody [EPR3813] (ab92390)
    All lanes : Anti-Pumilio 2 antibody [EPR3813] (ab92390) at 1/5000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 114 kDa
    Observed band size: 114 kDa

  • Anti-Pumilio 2 antibody [EPR3813] (ab92390)
    Anti-Pumilio 2 antibody [EPR3813] (ab92390)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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