Anti-Pumilio 2 antibody (ab190104)
Key features and details
- Rabbit polyclonal to Pumilio 2
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Pumilio 2 antibody
See all Pumilio 2 primary antibodies -
Description
Rabbit polyclonal to Pumilio 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Pumilio 2 conjugated to Keyhole Limpet Haemocyanin (KLH). surrounding S182. NP_056132.1.
Database link: Q8TB72-3 -
Positive control
- IHC-P: Human heart and Human prostate carcinoma tissue. Flow Cyt (Intra): HepG2 cells. WB: 293 cell lysate, transiently transfected with the Pumilio 2 gene.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Pumilio 2 antibody (ab190104) at 1/1000 dilution
Lane 1 : 293 cell lysate, non transfected
Lane 2 : 293 cell lysate, transiently transfected with the Pumilio 2 gene
Lysates/proteins at 2 µg per lane.
Predicted band size: 114 kDa
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling Pumilio 2 with ab190104 at 10 µg/ml followed by peroxidase-conjugated secondary antibody and DAB staining.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart labeling Pumilio 2 with ab190104 at 10 µg/ml.
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Flow cytometric analysis of HepG2 cells labeling Pumilio 2 with ab190104 at 1/10 dilution (lower image) compared with a negative control (upper image). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.