Anti-AHA1 antibody [25F2.D9] (ab91259)
![Anti-AHA1 antibody [25F2.D9] (ab91259)](https://www.abcam.com/ps/products/91/ab91259/Images/ab91259-199509-anti-aha1-antibody-25f2d9-western-blot.jpg "Anti-AHA1 antibody [25F2.D9] (ab91259)")
Key features and details
- Rat monoclonal [25F2.D9] to AHA1
- Suitable for: IHC-P, WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
-
Product name
Anti-AHA1 antibody [25F2.D9]
See all AHA1 primary antibodies -
Description
Rat monoclonal [25F2.D9] to AHA1 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseIP HumanWB Human
-
Immunogen
Mouse AHA1
-
Positive control
- ICC/IF: HeLa cells. IHC-P: Mouse back skin. IP: HeLa cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
25F2.D9 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
-
Western blot - Anti-AHA1 antibody [25F2.D9] (ab91259)Anti-AHA1 antibody [25F2.D9] (ab91259) at 1/1000 dilution + Human cell line lysate
Predicted band size: 38 kDa
-
![Immunocytochemistry/ Immunofluorescence - Anti-AHA1 antibody [25F2.D9] (ab91259)](https://www.abcam.com/ps/products/91/ab91259/Images/ab91259-106844-anti-aha1-antibody-25f2d9-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AHA1 antibody [25F2.D9] (ab91259)ab91259 staining AHA1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rat IgG secondary antibody was used. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHA1 antibody [25F2.D9] (ab91259)](https://www.abcam.com/ps/products/91/ab91259/Images/ab91259-310968-anti-aha1-antibody-25f2d9-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHA1 antibody [25F2.D9] (ab91259)Paraffin-embedded mouse back skin tissue stained for AHA1 using ab91259 at 1/100 dilution in immunohistochemical analysis. Secondary antibody is FITC Goat Anti-Rat.
-
![Immunoprecipitation - Anti-AHA1 antibody [25F2.D9] (ab91259)](https://www.abcam.com/ps/products/91/ab91259/Images/ab91259-106843-anti-aha1-antibody-25f2d9-immunoprecipitation.jpg)
Immunoprecipitation - Anti-AHA1 antibody [25F2.D9] (ab91259)ab91259 used in the Immunoprecipitation of AHA1 from Human Hela cells. The antibody was used at 20µl per 1 ml of cell lysate (50mM Tris (pH 7.2); 150mM NaCl; 1mM MgCl2; 1% TX-100; protease inhibitors); captured on 20µl of protein G beads .
