Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AHA1 antibody [25F2.D9] (ab91259)

Key features and details

  • Rat monoclonal [25F2.D9] to AHA1
  • Suitable for: IHC-P, WB, IP, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-AHA1 antibody [25F2.D9]
    See all AHA1 primary antibodies

  • Description

    Rat monoclonal [25F2.D9] to AHA1

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Mouse AHA1

  • Positive control

    • ICC/IF: HeLa cells. IHC-P: Mouse back skin. IP: HeLa cell lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    25F2.D9

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

Images

  • Western blot - Anti-AHA1 antibody [25F2.D9] (ab91259)
    Western blot - Anti-AHA1 antibody [25F2.D9] (ab91259)
    Anti-AHA1 antibody [25F2.D9] (ab91259) at 1/1000 dilution + Human cell line lysate

    Predicted band size: 38 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-AHA1 antibody [25F2.D9] (ab91259)
    Immunocytochemistry/ Immunofluorescence - Anti-AHA1 antibody [25F2.D9] (ab91259)
    ab91259 staining AHA1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rat IgG secondary antibody was used.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHA1 antibody [25F2.D9] (ab91259)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHA1 antibody [25F2.D9] (ab91259)

    Paraffin-embedded mouse back skin tissue stained for AHA1 using ab91259 at 1/100 dilution in immunohistochemical analysis. Secondary antibody is FITC Goat Anti-Rat.

  • Immunoprecipitation - Anti-AHA1 antibody [25F2.D9] (ab91259)
    Immunoprecipitation - Anti-AHA1 antibody [25F2.D9] (ab91259)
    ab91259 used in the Immunoprecipitation of AHA1 from Human Hela cells. The antibody was used at 20µl per 1 ml of cell lysate (50mM Tris (pH 7.2); 150mM NaCl; 1mM MgCl2; 1% TX-100; protease inhibitors); captured on 20µl of protein G beads .

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