Anti-Cytosolic Phospholipase A2 antibody (ab58375)
Key features and details
- Rabbit polyclonal to Cytosolic Phospholipase A2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytosolic Phospholipase A2 antibody
See all Cytosolic Phospholipase A2 primary antibodies -
Description
Rabbit polyclonal to Cytosolic Phospholipase A2 -
Host species
Rabbit -
Specificity
ab58375 detects endogenous levels of total Cytosolic Phospholipase A2 protein. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Cytosolic Phospholipase A2 (phospho S505).
Sequence:P-L-S-P-L
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab58375 was affinity purified from rabbit antiserum affinity chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cytosolic Phospholipase A2 antibody (ab58375)All lanes : Anti-Cytosolic Phospholipase A2 antibody (ab58375) at 1/500 dilution
Lane 1 : HeLa cell extracts treated
with TNF alpha (20ng/ml, 30mins)
Lane 2 : HeLa cell extracts treated
with TNF alpha (20ng/ml, 30mins) with immunising peptide
Predicted band size: 85 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody (ab58375)ab58375 at 1/50-1/100 dilution staining Cytosolic Phospholipase A2 in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytosolic Phospholipase A2 antibody (ab58375) This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of Human spleen tissue, staining Phospholipase A2 with ab58375.
Tissue was fixed with formaldehyde and blocked with blocking solution for 10 minutes at 37°C; antigen retrieval was by heat mediation in a cell conditioner. Samples were incubated with primary antibody (undiluted) for 1 hour at 37°C. A biotinylated-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
