Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246)
Key features and details
- Rabbit polyclonal to Cytosolic Phospholipase A2 - N-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cytosolic Phospholipase A2 antibody - N-terminal
See all Cytosolic Phospholipase A2 primary antibodies -
Description
Rabbit polyclonal to Cytosolic Phospholipase A2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Cow -
Immunogen
Recombinant fragment within Human Cytosolic Phospholipase A2 (N terminal). The exact sequence is proprietary.
Database link: P47712 -
Positive control
- IHC-P: Mouse duodenum and liver tissues; U87 xenograft tissue. WB: NIH/3T3 and Raji whole cell lysates. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246) at 1/1000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 85 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded mouse duodenum tissue stained for Cytosolic Phospholipase A2 using ab227246 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse liver tissue stained for Cytosolic Phospholipase A2 using ab227246 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded U87 xenograft tissue stained for Cytosolic Phospholipase A2 using ab227246 at 1/500 dilution in immunohistochemical analysis.
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Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cytosolic Phospholipase A2 (green) using ab227246 at 1/200 dilution in ICC/IF.
Lower panel: Merged with DNA probe.
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Anti-Cytosolic Phospholipase A2 antibody - N-terminal (ab227246) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Predicted band size: 85 kDa7.5% SDS-PAGE gel.