Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-12B antibody [EPR5739] (ab133752)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5739] to IL-12B
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-IL-12B antibody [EPR5739]
    See all IL-12B primary antibodies

  • Description

    Rabbit monoclonal [EPR5739] to IL-12B

  • Host species

    Rabbit

  • Specificity

    This antibody is unsuitable to detect endogenous IL-12B in WB.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human IL-12B aa 300-400. The exact sequence is proprietary.
    Database link: P29460

  • Positive control

    • WB: Human IL-12B recombinant protein. ICC/IF: MOLT-4 cells Flow Cyt: Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-12B antibody [EPR5739] (ab133752)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-12B antibody [EPR5739] (ab133752)

    Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling IL-12B with Purified ab133752 at 1:100 dilution (3.3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were not counterstained. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-IL-12B antibody [EPR5739] (ab133752)
    Western blot - Anti-IL-12B antibody [EPR5739] (ab133752)
    All lanes : Anti-IL-12B antibody [EPR5739] (ab133752) at 1/1000 dilution (Purified)

    All lanes : Human IL-12 recombinant protein

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?



    The recombinant protein contains Human IL-12B (Ile23-Ser328) and Human IL-12A (Arg23-Ser219) connected by disulfide bond to be heterodimer. Under reducing conditions, the observed bands will be at 40 KD for IL-12B and 29 KD for IL-12A.

  • Western blot - Anti-IL-12B antibody [EPR5739] (ab133752)
    Western blot - Anti-IL-12B antibody [EPR5739] (ab133752)
    Anti-IL-12B antibody [EPR5739] (ab133752) at 1/1000 dilution + Human IL-12B recombinant protein at 0.01 µg

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?



    This image was generated using the unpurified version of the product. 

  • Flow Cytometry - Anti-IL-12B antibody [EPR5739] (ab133752)
    Flow Cytometry - Anti-IL-12B antibody [EPR5739] (ab133752)

    Flow cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling IL-12B (red) with ab133752 at a 1/2500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

     

  • OI-RD Scanning - Anti-IL-12B antibody [EPR5739] (ab133752)
    OI-RD Scanning - Anti-IL-12B antibody [EPR5739] (ab133752)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-IL-12B antibody [EPR5739] (ab133752)
    Anti-IL-12B antibody [EPR5739] (ab133752)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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