Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24011-161] to IL37 - BSA and Azide free
  • Suitable for: WB, ICC, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-IL37 antibody [EPR24011-161] - BSA and Azide free
    See all IL37 primary antibodies

  • Description

    Rabbit monoclonal [EPR24011-161] to IL37 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, IHC-P, IPmore details
    Unsuitable for: Flow Cyt (Intra)

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate ; THP-1 treated with 100nM PMA for 16 hours. And then with 100 ng/ml LPS for 4 hours, and then 1 µg/ml Brefeldin A was added for 3 hours, whole cell lysate; Human testis tissue lysate; Human IL-37 recombinant protein. IHC-P: Human testis and skin. ICC: THP-1 cells. IP: THP-1 treated with 100nM PMA for 16 hours. And then with 100ng/ml LPS for 4 hours, and then 1µg/ml Brefeldin A was added for 3 hours whole cell lysate.

  • General notes

    ab278505 is the carrier-free version of ab278499. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278508 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)
    Western blot - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)
    All lanes : Anti-IL37 antibody [EPR24011-161] (ab278499) at 1/5000 dilution

    Lane 1 : Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate
    Lane 2 : THP-1 treated with 100nM PMA for 16 hours. And then replace PMA with /ml LPS(Lipopolysaccharide) for 4 hours, and then 1/ml Brefeldin A was added for the last 3 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab278499, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    IL 37 form homodimer through non-disulfide bonds. The molecular weight observed is consistent with what have been described in literature (PMID:20935647, 28783685).

    PMA and LPS treatment increase the expression of IL 37 (PMID:20935647).

    Exposure time: 48 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)

    This data was developed using ab278499, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling IL37 with ab278499 at 1/5000 (0.21 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human testis (PMID:11145836). The section was incubated with ab278499 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)
    Immunocytochemistry - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)

    This data was developed using ab278499, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 cells labelling IL37 with ab278499 at 1/250 (2.272 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong cytoplasmic and weak nuclear staining in THP-1 cells treated with phorbol 12-myristate 13-acetate (100nM) for 16 hours, and then with Lipopolysaccharide (100 ng/ml) for 4 hours and co-treated with brefeldin A (1ug/ml) for another 3 hours. PMA and LPS treatment increase the expression of IL 37 (PMID:20935647) is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunoprecipitation - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)
    Immunoprecipitation - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)

    This data was developed using ab278499, the same antibody clone in a different buffer formulation.

    IL37 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) treated with 100nM PMA for 16 hours. And then replace PMA with 100 ng/ml LPS (Lipopolysaccharide) for 4 hours, and then 1 ug/ml Brefeldin A was added for the last 3 hours, whole cell lysate 10 ug with ab278499 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab278499 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: THP-1 (human monocytic leukemia monocyte) treated with 100nM PMA for 16 hours. And then replace PMA with 100 ng/ml LPS(Lipopolysaccharide) for 4 hours, and then 1 ug/ml Brefeldin A was added for the last 3 hours, whole cell lysate 10 ug

    Lane 2: ab278499 IP in THP-1 treated with 100nM PMA for 16 hours. And then replace PMA with 100 ng/ml LPS(Lipopolysaccharide) for 4 hours, and then 1 ug/ml Brefeldin A was added for the last 3 hours whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab278499 in THP-1 treated with 100nM PMA for 16 hours. And then replace PMA with 100 ng/ml LPS(Lipopolysaccharide) for 4 hours, and then 1 ug/ml Brefeldin A was added for the last 3 hours whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

     

  • Western blot - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)
    Western blot - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)
    Anti-IL37 antibody [EPR24011-161] (ab278499) at 1/5000 dilution + Human IL-37 recombinant protein (aa46-218), 10 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 24 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?



    This data was developed using ab278499, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 10 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)

    This data was developed using ab278499, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling IL37 with ab278499 at 1/5000 (0.21 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on granular layer of human skin (PMID:24441097). The section was incubated with ab278499 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)
    Western blot - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)
    Anti-IL37 antibody [EPR24011-161] (ab278499) at 1/1000 dilution + Human testis tissue lysate at 20 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

    Predicted band size: 24 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This data was developed using ab278499, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    IL 37 form homodimer through non-disulfide bonds. The molecular weight observed is consistent with what have been described in literature (PMID:20935647, 28783685).

    Exposure time: 37 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL37 antibody [EPR24011-161] - BSA and Azide free (ab278505)

    This data was developed using ab278499, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human psoriasic skin tissue labeling IL37 with ab278499 at 1/5000 (0.21 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on human psoriasic skin (PMID:24441097). The section was incubated with ab278499 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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