Anti-TWEAKR/FN14 antibody [EPR23427-30] - BSA and Azide free (ab275381)
![Anti-TWEAKR/FN14 antibody [EPR23427-30] - BSA and Azide free (ab275381)](https://www.abcam.com/ps/products/275/ab275381/Images/ab275381-2-anti-tweakrfn14antibody-epr2342730-flow-cytometry-nih3t31.jpg "Anti-TWEAKR/FN14 antibody [EPR23427-30] - BSA and Azide free (ab275381)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23427-30] to TWEAKR/FN14 - BSA and Azide free
- Suitable for: Flow Cyt, IP
- Reacts with: Mouse
Overview
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Product name
Anti-TWEAKR/FN14 antibody [EPR23427-30] - BSA and Azide free
See all TWEAKR/FN14 primary antibodies -
Description
Rabbit monoclonal [EPR23427-30] to TWEAKR/FN14 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IPmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow cyt: NIH/3T3 cells (either starved for 8h then treated with 30ng/ml PMA for 16h or starved for 8h then treated with 10ng/ml FGF2 for 16h). IP: C2C12 whole cell lysate.
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General notes
ab275381 is the carrier-free version of ab275375. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab275381 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23427-30 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-TWEAKR/FN14 antibody [EPR23427-30] - BSA and Azide free (ab275381)
This data was developed using ab275375, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of NIH/3T3 (Mouse embryonic fibroblast) (starved for 8h then treated with 10ng/ml FGF2 for 16h) (Red) / Untreated control (Green) cells labelling TWEAKR/FN14 with ab275375 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR23427-30] - BSA and Azide free (ab275381)](https://www.abcam.com/ps/products/275/ab275381/Images/ab275381-3-anti-tweakrfn14antibody-epr2342730-immunoprecipitation-c2c12.jpg)
Immunoprecipitation - Anti-TWEAKR/FN14 antibody [EPR23427-30] - BSA and Azide free (ab275381)This data was developed using ab275375, the same antibody clone in a different buffer formulation.
TWEAKR/FN14 was immunoprecipitated from 0.35 mg C2C12 (mouse myoblasts myoblast) whole cell lysate with ab275375 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275375 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: C2C12 (mouse myoblasts myoblast) whole cell lysate 10 ug
Lane 2: ab275375 IP in C2C12 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275375 in C2C12 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 50 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID:17383968).
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![Flow Cytometry - Anti-TWEAKR/FN14 antibody [EPR23427-30] - BSA and Azide free (ab275381)](https://www.abcam.com/ps/products/275/ab275381/Images/ab275381-1-anti-tweakrfn14antibody-epr2342730-flow-cytometry-nih3t3.jpg)
Flow Cytometry - Anti-TWEAKR/FN14 antibody [EPR23427-30] - BSA and Azide free (ab275381)This data was developed using ab275375, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of NIH/3T3 (Mouse embryonic fibroblast) (starved for 8h then treated with 30ng/ml PMA for 16h) (Red) / Untreated control (Green) cells labelling TWEAKR/FN14 with ab275375 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Anti-TWEAKR/FN14 antibody [EPR23427-30] - BSA and Azide free (ab275381)](https://www.abcam.com/ps/products/275/ab275381/Images/ab275381-4-benefits-of-recombinant-antibodies.png)
Anti-TWEAKR/FN14 antibody [EPR23427-30] - BSA and Azide free (ab275381)