Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free (ab250595)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13171] to PDLIM7 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free
    See all PDLIM7 primary antibodies

  • Description

    Rabbit monoclonal [EPR13171] to PDLIM7 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen sequence for this antibody shares 86% homology with the mouse PDLIM7 protein however in our hands this antibody did not work in mouse and rat samples in western blot (no bands detected). In some human cell lines, in addition to the band of interest detected at 50 kDa, this product also bound to a protein at ~37-40 kDa in weight. For best results it may be preferable to use a harsh lysis protocol for preparation of cell or tissue lysates. Please see notes in the western blot image below.

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250595 is the carrier-free version of ab182153. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250595 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free (ab250595)
    Western blot - Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free (ab250595)
    Lane 1 : Anti-PDLIM7 antibody [EPR13171] (ab182153) at 1/10000 dilution
    Lanes 2-4 : Anti-PDLIM7 antibody [EPR13171] (ab182153) at 1/2000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 2 : Jurkat (human acute T cell leukemia) whole cell lysate
    Lane 3 : WI-38 (human lung) whole cell lysate
    Lane 4 : SK-OV-3 (human adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG (H+L) peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa



    This data was developed using ab182153, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: 

    Lane 1: 1 minute;

    Lanes 2 - 4: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free (ab250595)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free (ab250595)
    This data was developed using ab182153, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human infiltrating ductal breast carcinoma tissue labeling PDLIM7 with ab182153 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free (ab250595)
    Flow Cytometry - Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free (ab250595)
    This data was developed using ab182153, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PDLIM7 with ab182153 at 1/60 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
  • Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free (ab250595)
    Anti-PDLIM7 antibody [EPR13171] - BSA and Azide free (ab250595)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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