Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Serpin A5 antibody [EPR23337-69] - BSA and Azide free (ab272222)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23337-69] to Serpin A5 - BSA and Azide free
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Serpin A5 antibody [EPR23337-69] - BSA and Azide free
    See all Serpin A5 primary antibodies

  • Description

    Rabbit monoclonal [EPR23337-69] to Serpin A5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human serum, plasma and testis lysates; MCF7, HepG2 and MDA-MB-231 whole cell lysates. IHC-P: Human kidney and testis tissue. IP: Human serum lysate.

  • General notes

    ab272222 is the carrier-free version of ab270450. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-69] - BSA and Azide free (ab272222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-69] - BSA and Azide free (ab272222)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Serpin A5 with ab270450 at 1/5000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the human testis (PMID: 1372913). The section was incubated with ab270450 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270450).

  • Immunoprecipitation - Anti-Serpin A5 antibody [EPR23337-69] - BSA and Azide free (ab272222)
    Immunoprecipitation - Anti-Serpin A5 antibody [EPR23337-69] - BSA and Azide free (ab272222)

    Serpin A5 was immunoprecipitated from 0.35 mg Human serum lysate with ab270450 at 1/30 dilution . Western blot was performed on the immunoprecipitate using ab270450 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human serum lysate 10 ug

    Lane 2: ab270450 IP in Human serum lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270450 in Human serum lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270450).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-69] - BSA and Azide free (ab272222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serpin A5 antibody [EPR23337-69] - BSA and Azide free (ab272222)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Serpin A5 with ab270450 at 1/5000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the proximal tubular epithelium of the human kidney (PMID: 14696115). The section was incubated with ab270450 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270450).

  • Anti-Serpin A5 antibody [EPR23337-69] - BSA and Azide free (ab272222)
    Anti-Serpin A5 antibody [EPR23337-69] - BSA and Azide free (ab272222)

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