Anti-Tissue Plasminogen Activator antibody (ab227069)
Key features and details
- Rabbit polyclonal to Tissue Plasminogen Activator
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Tissue Plasminogen Activator antibody
See all Tissue Plasminogen Activator primary antibodies -
Description
Rabbit polyclonal to Tissue Plasminogen Activator -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human TPA Tissue Plasminogen Activator (internal sequence). The exact sequence is proprietary.
Database link: P00750 -
Positive control
- WB: Human plasma; HEK-293T, Neuro-2a and PC-12 whole cell lysates. IHC-P: Human colon carcinoma and hepatoma tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-Tissue Plasminogen Activator antibody (ab227069) at 1/2000 dilution + Human plasma at 30 µg
Predicted band size: 63 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded human colon carcinoma tissue stained for TPA Tissue Plasminogen Activator using ab227069 at 1/250 dilution in immunohistochemical analysis.
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Paraffin-embedded human hepatoma tissue stained for TPA Tissue Plasminogen Activator using ab227069 at 1/500 dilution in immunohistochemical analysis.
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Anti-Tissue Plasminogen Activator antibody (ab227069) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 63 kDa7.5% SDS PAGE gel.
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Anti-Tissue Plasminogen Activator antibody (ab227069) at 1/1000 dilution + Neuro 2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 63 kDa7.5% SDS-PAGE gel.
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Anti-Tissue Plasminogen Activator antibody (ab227069) at 1/1000 dilution + PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 63 kDa7.5% SDS-PAGE gel.