Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7232(2)] to Tissue Plasminogen Activator
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Tissue Plasminogen Activator antibody [EPR7232(2)]
    See all Tissue Plasminogen Activator primary antibodies

  • Description

    Rabbit monoclonal [EPR7232(2)] to Tissue Plasminogen Activator

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    corresponding to Human TPA Tissue Plasminogen Activator.

  • Positive control

    • WB: Mouse plasma, Rat plasma, Human plasma, and Human liver lysates; IHC-P: human lung carcinoma, cervical carcinoma, skeletal muscle, brain, kidney and liver tissue, mouse and rat liver tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
    Western blot - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
    All lanes : Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469) at 1/1000 dilution (Purified)

    Lane 1 : Mouse plasma lysates
    Lane 2 : Rat plasma lysates
    Lane 3 : Human plasma lysates
    Lane 4 : Human brain lysates
    Lane 5 : Human heart lysates
    Lane 6 : Human liver lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 63 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?



    T-PA is serected and mainly released into blood and extracellular fluid to activate plasminogen. The molecular weight observed is consistent with what has been described in PMID: 22069469

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling TPA Tissue Plasminogen Activator with purified ab157469 at 1/1000 dilution (1.91 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)

    ab157469 (unpurified) showing +ve staining in Human normal liver.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)

    ab157469 (unpurified) showing +ve staining in Human normal kidney.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)

    ab157469 (unpurified) showing +ve staining in Human normal brain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)

    ab157469 (unpurified) showing +ve staining in Human skeletal muscle.

  • Western blot - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
    Western blot - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
    Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469) at 1/1000 dilution (unpurified) + Human plasma lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 63 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)

    Immunohistochemical analysis of paraffin-embedded Human cervial carcinoma tissue labeling TPA Tissue Plasminogen Activator with ab157469 (unpurified) 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TPA Tissue Plasminogen Activator with ab157469  (unpurified) at 1/100 dilution.

  • Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
    Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)

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