Anti-Tissue Plasminogen Activator antibody [H27B6] (ab28374)
Key features and details
- Mouse monoclonal [H27B6] to Tissue Plasminogen Activator
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Tissue Plasminogen Activator antibody [H27B6]
See all Tissue Plasminogen Activator primary antibodies -
Description
Mouse monoclonal [H27B6] to Tissue Plasminogen Activator -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Recombinant full length protein (Mouse)
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Epitope
protease domain (see Declerck et al) -
Positive control
- This antibody gave a positive result in IHC in the following FFPE tissue: Rat Normal Kidney.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 0.82% Sodium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Protein A Sepharose. Purity is = 98% assessed by visual inspection of a Coomassie® bluestained SDS-PAGE gel. -
Clonality
Monoclonal -
Clone number
H27B6 -
Isotype
IgG -
Research areas
Images
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IHC image of TPA Tissue Plasminogen Activator staining in Rat Normal Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28374, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.