Angiotensin A ELISA Kit (ab136935)
Key features and details
- Sensitivity: 8.3 pg/ml
- Range: 9.8 pg/ml - 10000 pg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Angiotensin A ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Buffer 20 1297.9pg/ml 7.5% Buffer 20 655.4pg/ml 4.3% Buffer 20 55.2pg/ml 10.3% Inter-assay Sample n Mean SD CV% Buffer 1248.2pg/ml 10.9% Buffer 612.2pg/ml 5.2% Buffer 55.6pg/ml 17.7% -
Sample type
Serum, Plasma -
Assay type
Competitive -
Sensitivity
8.3 pg/ml -
Range
9.8 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94 50pg/ml - 5000pg/ml Plasma 109 50pg/ml - 5000pg/ml -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Angiotensin A in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Angiotensin A in plasma and serum.
A goat anti-rabbit IgG polyclonal antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with a solution of Angiotensin A conjugated to biotin, followed by a solution of polyclonal antibody to Angiotensin A. The plate is washed to remove unbound reagents. A solution of streptavidin-HRP conjugate is then added. After further incubation the excess reagents are washed away and TMB substrate is added, which is catalyzed by HRP to generate a yellow color. A stop solution changes this color from yellow to blue, and the intensity of this blue coloration is inversely proportional to the amount of Angiotensin A captured in the plate.
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Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Angiotensin A Antibody 1 vial Angiotensin A Biotin Conjugate 1 vial Angiotensin A Standard 2 x 40ng Assay Buffer 16 1 x 30ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit HRP- Streptavidin Conjugate 1 x 12.5µg Plate Sealer 2 units Stop Solution 2 1 x 10ml TMB Substrate 2 x 10ml -
Research areas
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Alternative names
- AGT
- ANGT_HUMAN
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Database links
- Entrez Gene: 183 Human
- Omim: 106150 Human
- SwissProt: P01019 Human
- Unigene: 19383 Human
Images
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Typical Standard Curve
Representative standard curve using ab136935.
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Typical Parallelism CurveDose‐response curves from human plasma and serum diluted into assay buffer were compared to the Angiotensin A standard curve. The parallel response indicates the standard effectively mimics the native protein.