Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Friday March 05, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8252] to GRAF
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-GRAF antibody [EPR8252]
See all GRAF primary antibodies
Description

Rabbit monoclonal [EPR8252] to GRAF
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human GRAF aa 750-850. The exact sequence is proprietary.
Positive control
- Human fetal heart, HeLa, MCF7 and fetal brain lysates; Human brain tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8252
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody [EPR8252] (ab137085)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling GRAF with ab137085 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-GRAF antibody [EPR8252] (ab137085)

All lanes : Anti-GRAF antibody [EPR8252] (ab137085) at 1/1000 dilution

Lane 1 : Human fetal heart lysates
Lane 2 : HeLa cell lysates
Lane 3 : MCF7 cell lysates
Lane 4 : Human fetal brain lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 92 kDa
Anti-GRAF antibody [EPR8252] (ab137085)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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