Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GRAF antibody [EPR8252] (ab137085)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8252] to GRAF
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-GRAF antibody [EPR8252]
    See all GRAF primary antibodies

  • Description

    Rabbit monoclonal [EPR8252] to GRAF

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human GRAF aa 750-850. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart, HeLa, MCF7 and fetal brain lysates; Human brain tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody [EPR8252] (ab137085)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRAF antibody [EPR8252] (ab137085)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling GRAF with ab137085 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-GRAF antibody [EPR8252] (ab137085)
    Western blot - Anti-GRAF antibody [EPR8252] (ab137085)
    All lanes : Anti-GRAF antibody [EPR8252] (ab137085) at 1/1000 dilution

    Lane 1 : Human fetal heart lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : MCF7 cell lysates
    Lane 4 : Human fetal brain lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 92 kDa

  • Anti-GRAF antibody [EPR8252] (ab137085)
    Anti-GRAF antibody [EPR8252] (ab137085)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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