Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - BSA and Azide free (ab235392)
![Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - BSA and Azide free (ab235392)](https://www.abcam.com/ps/products/235/ab235392/Images/ab235392-310463-ab75876DotBlot.jpg "Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - BSA and Azide free (ab235392)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2288Y] to Caveolin-1 (phospho Y14) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
-
Product name
Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - BSA and Azide free
See all Caveolin-1 primary antibodies -
Description
Rabbit monoclonal [EPR2288Y] to Caveolin-1 (phospho Y14) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects Caveolin-1 phosphorylated at tyrosine 14.
-
Tested applications
Suitable for: Dot blot, WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: A673, Insulin treated A673, A673 treated with insulin then the membrane was incubated with phosphatase, HeLa and HeLa cell treated with pervanadate whole cell lysate.
-
General notes
ab235392 is the carrier-free version of ab75876 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab235392 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2288Y -
Isotype
IgG -
Research areas
Images
-
Dot Blot - Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - BSA and Azide free (ab235392)
This Dot Blot data was generated using the same anti-Caveolin-1 (phospho Y14) antibody clone, EPR2288Y, in a different buffer formulation (cat# ab75876).
Dot blot analysis of Caveolin-1 (phospho Y14) phospho peptide (Lane 1), Caveolin-1 Non-phospho peptide (Lane 2) labelling Caveolin-1 (phospho Y14) with ab75876 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
-
![Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - BSA and Azide free (ab235392)](https://www.abcam.com/ps/products/235/ab235392/Images/ab235392-2-benefits-of-recombinant-antibodies.png)
Anti-Caveolin-1 (phospho Y14) antibody [EPR2288Y] - BSA and Azide free (ab235392)
