Alexa Fluor® 555 Anti-Cortactin antibody [EP1922Y] (ab215298)
![Alexa Fluor® 555 Anti-Cortactin antibody [EP1922Y] (ab215298)](https://www.abcam.com/ps/products/215/ab215298/Images/ab215298-271273-anti-cortactin-antibody-ep1922y-alexa-fluor-555-immunofluorescence.jpg "Alexa Fluor® 555 Anti-Cortactin antibody [EP1922Y] (ab215298)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 555 Rabbit monoclonal [EP1922Y] to Cortactin
- Suitable for: ICC/IF
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Overview
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Product name
Alexa Fluor® 555 Anti-Cortactin antibody [EP1922Y]
See all Cortactin primary antibodies -
Description
Alexa Fluor® 555 Rabbit monoclonal [EP1922Y] to Cortactin -
Host species
Rabbit -
Conjugation
Alexa Fluor® 555. Ex: 555nm, Em: 565nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HeLa and wildtype HAP1 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1922Y -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cortactin antibody [EP1922Y] (ab215298)
ab215298 staining Cortactin in wild-type HAP1 cells (top panel) and Cortactin in wild-type HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab215298 at 1/500 dilution (shown in pseudo colour green) and ab7291 at 1μg/ml concentration overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 647) (ab150119) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
This product also gave a positive signal under the same testing conditions in HAP1 cells fixed with 4% formaldehyde (10 min).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8)
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cortactin antibody [EP1922Y] (ab215298)](https://www.abcam.com/ps/products/215/ab215298/Images/ab215298-269040-anti-cortactin-antibody-ep1922y-alexa-fluor-555-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-Cortactin antibody [EP1922Y] (ab215298)Ab215298 staining Cortactin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab215298 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).
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![Alexa Fluor® 555 Anti-Cortactin antibody [EP1922Y] (ab215298)](https://www.abcam.com/ps/products/215/ab215298/Images/ab215298-3-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 555 Anti-Cortactin antibody [EP1922Y] (ab215298)
