Anti-Cortactin (phospho Y421) antibody (ab47768)
Key features and details
- Rabbit polyclonal to Cortactin (phospho Y421)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cortactin (phospho Y421) antibody
See all Cortactin primary antibodies -
Description
Rabbit polyclonal to Cortactin (phospho Y421) -
Host species
Rabbit -
Specificity
This antibody detects Cortactin only when phosphorylated at tyrosine 421. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthesized phosphopeptide derived from human Cortactin around the phosphorylation site of tyrosine 421 (P-I-YP-E-D).
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Positive control
- Hela cell extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cortactin (phospho Y421) antibody (ab47768)All lanes : Anti-Cortactin (phospho Y421) antibody (ab47768) at 1/500 dilution
Lane 1 : Hela cell extract, treated with H2O2
Lane 2 : Hela cell extract, untreated
Predicted band size: 62 kDa
Typically 5-30ug of total protein was loaded per lane of the gel. The cells were treated with 150 uM H2O2.
