Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14212(B)] to D4 GDI - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt (Intra), WB
  • Reacts with: Human

Overview

  • Product name

    Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free
    See all D4 GDI primary antibodies

  • Description

    Rabbit monoclonal [EPR14212(B)] to D4 GDI - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cyt (Intra), WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250465 is the carrier-free version of ab181252.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)
    Western blot - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)
    All lanes : Anti-D4 GDI antibody [EPR14212(B)] (ab181252) at 1/10000 dilution

    Lane 1 : Jurkat lysate
    Lane 2 : U937 lysate
    Lane 3 : HL60 lysate
    Lane 4 : Human spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Additional bands at: 23 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab181252, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)
    Flow Cytometry - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)
    This data was developed using ab181252, the same antibody clone in a different buffer formulation.Flow Cytometrical analysis of HL60 cells labeling D4 GDI with ab181252 at 1/70 (red) compared to a negative control antibody (green).
  • Immunocytochemistry - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)
    Immunocytochemistry - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)
    This data was developed using ab181252, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HL60 cells labeling D4 GDI with ab181252 at 1/100 (green) with Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 and DAPI staining (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)
    This data was developed using ab181252, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human bone marrow tissue labeling D4 GDI with ab181252 at 1/500. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)
    This data was developed using ab181252, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling D4 GDI with ab181252 at 1/500. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)
    Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)

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