Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)
![Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)](https://www.abcam.com/ps/products/250/ab250465/Images/ab250465-445613-anti-d4-gdi-antibody-epr14212-b-bsa-and-azide-free-western-blot.jpg "Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14212(B)] to D4 GDI - BSA and Azide free
- Suitable for: IHC-P, ICC, Flow Cyt (Intra), WB
- Reacts with: Human
Overview
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Product name
Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free
See all D4 GDI primary antibodies -
Description
Rabbit monoclonal [EPR14212(B)] to D4 GDI - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250465 is the carrier-free version of ab181252.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14212(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)All lanes : Anti-D4 GDI antibody [EPR14212(B)] (ab181252) at 1/10000 dilution
Lane 1 : Jurkat lysate
Lane 2 : U937 lysate
Lane 3 : HL60 lysate
Lane 4 : Human spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Additional bands at: 23 kDa. We are unsure as to the identity of these extra bands.This data was developed using ab181252, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)](https://www.abcam.com/ps/products/250/ab250465/Images/ab250465-4-anti-d4-gdi-antibody-epr14212-b-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465) -
![Immunocytochemistry - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)](https://www.abcam.com/ps/products/250/ab250465/Images/ab250465-3-anti-d4-gdi-antibody-epr14212-b-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)](https://www.abcam.com/ps/products/250/ab250465/Images/ab250465-2-anti-d4-gdi-antibody-epr14212-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)This data was developed using ab181252, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human bone marrow tissue labeling D4 GDI with ab181252 at 1/500. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)](https://www.abcam.com/ps/products/250/ab250465/Images/ab250465-1-anti-d4-gdi-antibody-epr14212-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)This data was developed using ab181252, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling D4 GDI with ab181252 at 1/500. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)](https://www.abcam.com/ps/products/250/ab250465/Images/ab250465-5-benefits-of-recombinant-antibodies.png)
Anti-D4 GDI antibody [EPR14212(B)] - BSA and Azide free (ab250465)
