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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins

Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (ab249510)

Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (ab249510)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10274] to ARPC5L - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB, Flow Cyt (Intra)
  • Reacts with: Human

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Overview

  • Product name

    Anti-ARPC5L antibody [EPR10274] - BSA and Azide free
    See all ARPC5L primary antibodies
  • Description

    Rabbit monoclonal [EPR10274] to ARPC5L - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC, WB, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ARPC5L. The exact sequence is proprietary.
    Database link: Q9BPX5

  • General notes

    ab249510 is the carrier-free version of ab169763.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10274
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins

Images

  • Flow Cytometry - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (ab249510)
    Flow Cytometry - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (ab249510)
    This data was developed using ab169763, the same antibody clone in a different buffer formulation.Flow Cytometrical analysis of permeabilized MOLT4 cells with ab169763 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
  • Immunocytochemistry - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (ab249510)
    Immunocytochemistry - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (ab249510)
    This data was developed using ab169763, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of MCF7 cells labeling ARPC5 with ab169763 at 1/250 (red) with DAPI staining (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (ab249510)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (ab249510)
    This data was developed using ab169763, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human heart tissue labeling ARPC5L with ab169763 at 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (ab249510)
    Western blot - Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (ab249510)
    All lanes : Anti-ARPC5L antibody [EPR10274] (ab169763) at 1/1000 dilution

    Lane 1 : U87-MG lysate
    Lane 2 : MCF7 lysate
    Lane 3 : MOLT4 lysate
    Lane 4 : Fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 17 kDa



    This data was developed using ab169763, the same antibody clone in a different buffer formulation.

     

  • Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (ab249510)
    Anti-ARPC5L antibody [EPR10274] - BSA and Azide free (ab249510)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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