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Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16310] to SCIN - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SCIN antibody [EPR16310] - C-terminal
    See all SCIN primary antibodies

  • Description

    Rabbit monoclonal [EPR16310] to SCIN - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal kidney lysate. Mouse and Rat kidneyand spleen lysates. HEK293, C6, PC-12 and NIH/3T3 whole cell lysates. IHC-P: Human clear cell carcinoma of kidney and Human pancreas tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)
    Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)
    All lanes : Anti-SCIN antibody [EPR16310] - C-terminal (ab199723) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : HEK293 (Human embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa


    Exposure time: 30 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)
    Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)
    All lanes : Anti-SCIN antibody [EPR16310] - C-terminal (ab199723) at 1/1000 dilution

    Lane 1 : Mouse kidney lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa


    Exposure time: 1 minute


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)
    Western blot - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)
    All lanes : Anti-SCIN antibody [EPR16310] - C-terminal (ab199723) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor cells) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa


    Exposure time: 5 seconds


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)

    Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling SCIN with ab199723 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human clear cell carcinoma of kidney tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SCIN with ab199723 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human pancreas tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)
    Anti-SCIN antibody [EPR16310] - C-terminal (ab199723)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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