Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18054] to Cyclin A1 + Cyclin A2
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054]
    See all Cyclin A1 + Cyclin A2 primary antibodies

  • Description

    Rabbit monoclonal [EPR18054] to Cyclin A1 + Cyclin A2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant fragment of Human Cyclin A1 protein; Full length Human Cyclin A2 recombinant protein; Human testis lysate. HeLa and HepG2 whole cell lysate. IHC-P: Human testis and colon cancer tissues. IP: K562 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18054

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)
    Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)
    Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/20000 dilution + Recombinant fragment of Human Cyclin A1 protein at 0.01 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 52, 49 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 seconds


    Recombinant fragment of Human Cyclin A1 protein contains aa261-450 with His-Tag & GST-tag.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Cyclin A1 + Cyclin A2 using ab185619 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining of spermatocytes of the human testis is observed. Counterstained with Hematoxylin.
    Negative control obtained using PBS instead of ab185619 and secondary antibody only.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)
    Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)
    Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/2000 dilution + Full length Human Cyclin A2 recombinant protein at 0.01 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 52, 49 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Recombinant full length Cyclin A2 protein contains aa1-432 with His-Tag.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)

    Immunohistochemical analysis of paraffin-embedded human colon cancer, labeling Cyclin A1 + Cyclin A2 with ab185619 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weak cytoplasmic staining of human colonic adenocarcinoma cells is observed. Counterstained with Hematoxylin.
    Negative control obtained using PBS instead of ab185619 and secondary antibody only.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)
    Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)
    Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/1000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 52, 49 kDa
    Observed band size: 49,52 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunoprecipitation - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)
    Immunoprecipitation - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)

    Cyclin A1 + Cyclin A2 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab185619 at 1/70 dilution. Western blot was performed from the immunoprecipitate using ab185619 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: K562 whole cell extract 10 µg (Input).

    Lane 2: ab185619 IP in K562 whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab185619 in K562 whole cell extract.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds

  • Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)
    Western blot - Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)
    All lanes : Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 52, 49 kDa
    Observed band size: 49,52 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)
    Anti-Cyclin A1 + Cyclin A2 antibody [EPR18054] (ab185619)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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