Anti-Cyclin A1 antibody [3F6B2C2] - BSA and Azide free (ab276044)
![Anti-Cyclin A1 antibody [3F6B2C2] - BSA and Azide free (ab276044)](https://www.abcam.com/ps/products/276/ab276044/Images/ab276044-2-AntiCyclin-A1-antibody-3F6B2C2-wb-THP1-HeLa-cells.jpg "Anti-Cyclin A1 antibody [3F6B2C2] - BSA and Azide free (ab276044)")
Key features and details
- Mouse monoclonal [3F6B2C2] to Cyclin A1 - BSA and Azide free
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Cyclin A1 antibody [3F6B2C2] - BSA and Azide free
See all Cyclin A1 primary antibodies -
Description
Mouse monoclonal [3F6B2C2] to Cyclin A1 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
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Immunogen
Recombinant full length protein (His-tag) corresponding to Human Cyclin A1 aa 1-465 (N terminal). NP_003905.1
Database link: P78396 -
Positive control
- ICC: HeLa cells. WB: THP-1 and HeLa whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3F6B2C2 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-Cyclin A1 antibody [3F6B2C2] (ab276044)All lanes : Anti-Cyclin A1 antibody [3F6B2C2] - BSA and Azide free (ab276044) at 1/500 dilution
Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution
Predicted band size: 52 kDaDeveloped using the ECL technique.
Performed under reducing conditions. -
![Immunocytochemistry - Anti-Cyclin A1 antibody [3F6B2C2] (ab276044)](https://www.abcam.com/ps/products/276/ab276044/Images/ab276044-1-AntiCyclin-A1-antibody-3F6B2C2-immunofluorescence-HeLa-cells.jpg)
Immunocytochemistry - Anti-Cyclin A1 antibody [3F6B2C2] (ab276044)Immunofluorescence staining of Cyclin A1 in HeLa (human epithelial cell line from cervix adenocarcinoma) cells. Cells were fixed with 4% PFA, permeabilized with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab276044 at 1/300 dilution at 37°C for 1 hour. Then cells were stained with an AlexaFluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
