Anti-DIAPH2/DIA antibody (ab230804)
Key features and details
- Rabbit polyclonal to DIAPH2/DIA
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DIAPH2/DIA antibody
See all DIAPH2/DIA primary antibodies -
Description
Rabbit polyclonal to DIAPH2/DIA -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human DIAPH2/DIA aa 870-899 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: O60879 -
Positive control
- WB: HEK-293 and MDS-MB-345 cell lysates. IHC-P: Human colon tissue. Flow Cyt: CEM cells.
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General notes
This product was previously labelled as DIAPH2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DIAPH2/DIA antibody (ab230804)All lanes : Anti-DIAPH2/DIA antibody (ab230804) at 1/1000 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : MDA-MB-435 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 126 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH2/DIA antibody (ab230804)Formalin-fixed, paraffin-embedded human colon tissue stained for DIAPH2/DIA using ab230804 at 1/75 dilution in immunohistochemical analysis.
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Flow Cytometry - Anti-DIAPH2/DIA antibody (ab230804)Flow cytometric analysis of CEM cells labeling DIAPH2/DIA with ab230804 at 1/10 dilution (green) compared with a negative control (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used.
