Anti-DIAPH2/DIA antibody (ab12319)
Key features and details
- Rabbit polyclonal to DIAPH2/DIA
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DIAPH2/DIA antibody
See all DIAPH2/DIA primary antibodies -
Description
Rabbit polyclonal to DIAPH2/DIA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human DIAPH2/DIA aa 50-100. The exact sequence is proprietary.
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Positive control
- HEK 293T cells.
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General notes
This product was previously labelled as DIAPH2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DIAPH2/DIA antibody (ab12319)
Detection of Human Diaphanous 2 by Western Blot.
Samples: Whole cell lysate (50 mcg) from HEK 293T cells.
Antibody: ab12319 used at the indicated concentrations.
Detection: Chemiluminescence.
Detection of Human Diaphanous 2 by Western Blot.
Samples: Whole cell lysate (50 mcg) from HEK 293T cells.
Antibody: ab12319 used at the indicated concentrations.
Detection: Chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIAPH2/DIA antibody (ab12319)IHC image of ab12319 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12319, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
