Anti-Vinculin (phospho Y822) antibody (ab200825)
Key features and details
- Rabbit polyclonal to Vinculin (phospho Y822)
- Suitable for: WB
- Isotype: IgG
Overview
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Product name
Anti-Vinculin (phospho Y822) antibody
See all Vinculin primary antibodies -
Description
Rabbit polyclonal to Vinculin (phospho Y822) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Predicted to work with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Vinculin (phospho Y822). chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 822.
Database link: P18206 -
Positive control
- Chick Embryo Fibroblasts (CEFs) transfected with activated Src.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab200825 has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated vinculin. The final product is generated by affinity chromatography using a vinculin-derived peptide that is phosphorylated at tyrosine 822. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Vinculin (phospho Y822) antibody (ab200825) at 1/1000 dilution
Lane 1 : Untransfected CEF lysates
Lane 2 : Src transfected CEF lysates
Lane 3 : Src transfected CEF lysates with non-phosphopeptide corresponding to immunogen
Lane 4 : Src transfected CEF lysates with generic phosphotyrosine containing peptide
Lane 5 : Src transfected CEF lysates with phosphopeptide immunogen
Secondary
Lanes 1-2 : goat F(ab’)2 anti-rabbit IgG HRP conjugate
Developed using the ECL technique.
Predicted band size: 124 kDa10% polyacrylamide gel transferred to PVDF blocked with a 5% BSA-TBST