Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Vinculin antibody [EPR8185] (ab129002)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8185] to Vinculin
  • Suitable for: Flow Cyt, WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Vinculin antibody [EPR8185]
    See all Vinculin primary antibodies

  • Description

    Rabbit monoclonal [EPR8185] to Vinculin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Vinculin aa 1000-1100. The exact sequence is proprietary.
    Database link: P18206

  • Positive control

    • WB: PC3, HeLa, U937, K562, HUVEC, HepG2 human fetal liver and human fetal kidney lysates ICC/IF: HUVEC cells, HEK-293 cells. IP: HeLa cells Flow Cyt: HEK293 cells.

  • General notes

      

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] (ab129002)
    Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] (ab129002)

    Immunofluorescent staining of HEK293 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab129002 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) was used as the secondary at a dilution of 1/1000 and the cells were counter stained with DAPI. The negative controls are shown in the bottom middle and right hand panels. For negative control 1, the primary was used and then goat anti-mouse IgG was used at a dilution of 1/500. For negative control 2, a mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) were used.

  • Western blot - Anti-Vinculin antibody [EPR8185] (ab129002)
    Western blot - Anti-Vinculin antibody [EPR8185] (ab129002)
    All lanes : Anti-Vinculin antibody [EPR8185] (ab129002) at 1/10000 dilution (purified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : PC3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 124 kDa
    Observed band size: 124 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Flow Cytometry - Anti-Vinculin antibody [EPR8185] (ab129002)
    Flow Cytometry - Anti-Vinculin antibody [EPR8185] (ab129002)

    Flow cytometry analysis of 293 (human embryonic kidney epithelial) cells labeling Vinculin (red) with ab129002 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Immunoprecipitation - Anti-Vinculin antibody [EPR8185] (ab129002)
    Immunoprecipitation - Anti-Vinculin antibody [EPR8185] (ab129002)

    ab129002 (purified) at 1/20 immunoprecipitating vinculin in HeLa cells. Lane 1: HeLa whole cell lysate (10 µg). Lane 2: HeLa whole cell lysate (10 µg). Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab129002 in HeLa whole cell lysate. For western blotting, a HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-Vinculin antibody [EPR8185] (ab129002)
    Western blot - Anti-Vinculin antibody [EPR8185] (ab129002)
    All lanes : Anti-Vinculin antibody [EPR8185] (ab129002) at 1/20000 dilution (purified)

    Lane 1 : C6 cell lysate
    Lane 2 : rat kidney cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 124 kDa
    Observed band size: 124 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] (ab129002)
    Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] (ab129002)

    Immunofluorescent staining of vinculin in HUVEC cells with unpurified ab129002 at 1/100 dilution.

  • Western blot - Anti-Vinculin antibody [EPR8185] (ab129002)
    Western blot - Anti-Vinculin antibody [EPR8185] (ab129002)
    All lanes : Anti-Vinculin antibody [EPR8185] (ab129002) at 1/10000 dilution (purified)

    Lane 1 : mouse spleen
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 124 kDa
    Observed band size: 124 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Vinculin antibody [EPR8185] (ab129002)
    Western blot - Anti-Vinculin antibody [EPR8185] (ab129002)
    All lanes : Anti-Vinculin antibody [EPR8185] (ab129002) at 1/10000 dilution (unpurified)

    Lane 1 : PC3 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : U937 cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : HUVEC cell lysate
    Lane 6 : Human fetal liver lysate
    Lane 7 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 124 kDa
    Observed band size: 124 kDa

  • Anti-Vinculin antibody [EPR8185] (ab129002)
    Anti-Vinculin antibody [EPR8185] (ab129002)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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