Anti-Vinculin (phospho Y100) antibody (ab200812)
Key features and details
- Rabbit polyclonal to Vinculin (phospho Y100)
- Suitable for: WB
- Reacts with: Chicken
- Isotype: IgG
Overview
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Product name
Anti-Vinculin (phospho Y100) antibody
See all Vinculin primary antibodies -
Description
Rabbit polyclonal to Vinculin (phospho Y100) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Chicken
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Immunogen
Synthetic peptide corresponding to Human Vinculin (phospho Y100). derived from a region that contains tyrosine 100.
Database link: P18206 -
Positive control
- COS cells co-transfected with activated Src and His-tagged chicken Vinculin cDNA were treated with vanadate for 24 hr.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Vinculin (phospho Y100) antibody (ab200812)Lanes 1-5 : Anti-Vinculin (phospho Y100) antibody (ab200812) at 1/1000 dilution
Lanes 6-10 : Anti-Vinculin pan antibody
Lane 1 : COS cells co-transfected with activated Src and His-tagged chicken Vinculin cDNA untreated
Lanes 2 & 7-10 : COS cells co-transfected with activated Src and His-tagged chicken Vinculin cDNA were treated with vanadate for 24 hr
Lane 3 : COS cells co-transfected with activated Src and His-tagged chicken Vinculin cDNA were treated with vanadate for 24 hr. with non-phosphopeptide corresponding to the immunogen
Lane 4 : COS cells co-transfected with activated Src and His-tagged chicken Vinculin cDNA were treated with vanadate for 24 hr with generic phosphotyrosine-containing peptide
Lane 5 : COS cells co-transfected with activated Src and His-tagged chicken Vinculin cDNA were treated with vanadate for 24 hr with phosphopeptide immunogen
Lane 6 : COS cells co-transfected with activated Src and His-tagged chicken Vinculin cDNA were untreated
Developed using the ECL technique.
Predicted band size: 124 kDaFollowing immunoprecipitation of Vinculin with an anti-His monoclonal antibody, proteins were resolved by SDS-PAGE on an 8% polyacrylamide gel and transferred to PVDF.
