Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Vinculin antibody [EPR19579] (ab207440)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19579] to Vinculin
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-Vinculin antibody [EPR19579]
See all Vinculin primary antibodies
Description

Rabbit monoclonal [EPR19579] to Vinculin
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: K562, HeLa, HEK-293T, HUVEC, MCF7, PC-3, C6, PC-12, A431, and NIH/3T3 whole cell lysates; human testis, fetal heart and fetal kidney lysates; mouse heart, kidney and spleen lysates; rat kidney and spleen lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19579
Isotype

IgG
Research areas

Western blot - Anti-Vinculin antibody [EPR19579] (ab207440)

All lanes : Anti-Vinculin antibody [EPR19579] (ab207440) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : Vinculin knockout HeLa cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 124 kDa

Lanes 1-4: Merged signal (red and green). Green - ab207440 observed at 124 kDa. Red - loading control ab7291 observed at 50 kDa.

ab207440 Anti-Vinculin antibody [EPR19579] was shown to specifically react with Vinculin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265580 (knockout cell lysate ab257795) was used. Wild-type and Vinculin knockout samples were subjected to SDS-PAGE. ab207440 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Vinculin antibody [EPR19579] (ab207440)

All lanes : Anti-Vinculin antibody [EPR19579] (ab207440) at 1/1000 dilution

Lane 1 : Wild-type A431 whole cell lysate
Lane 2 : VCL knockout A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 124 kDa
Observed band size: 124 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab207440 observed at 124 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab207440 was shown to react with VCL in A431 wild-type cells in Western blot. Loss of signal was observed when VCL knockout sample was used. A431 wild-type and VCL knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab207440 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Vinculin antibody [EPR19579] (ab207440)

All lanes : Anti-Vinculin antibody [EPR19579] (ab207440) at 1/2000 dilution

Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 6 : Human testis lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 124 kDa
Observed band size: 124 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1-4: 3 minutes; Lane 5/6: 5 seconds.
Western blot - Anti-Vinculin antibody [EPR19579] (ab207440)

All lanes : Anti-Vinculin antibody [EPR19579] (ab207440) at 1/1000 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 124 kDa
Observed band size: 124 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Vinculin antibody [EPR19579] (ab207440)

All lanes : Anti-Vinculin antibody [EPR19579] (ab207440) at 1/1000 dilution

Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat kidney lysate
Lane 5 : Rat spleen lysate
Lane 6 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 8 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 124 kDa
Observed band size: 124 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1-3: 30 seconds; Lane 4-8: 10 seconds.
Anti-Vinculin antibody [EPR19579] (ab207440)

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