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Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)

Key features and details

  • Mouse monoclonal [VCL/2575] to Vinculin - BSA and Azide free
  • Suitable for: WB, IHC-P, Protein Array
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-Vinculin antibody [VCL/2575] - BSA and Azide free
    See all Vinculin primary antibodies

  • Description

    Mouse monoclonal [VCL/2575] to Vinculin - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, Protein Arraymore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment within Human Vinculin aa 174-322. The exact sequence is proprietary.
    Database link: P18206

  • Positive control

    • WB: A431, K562 and NIH/3T3 cell lysate. IHC-P: Human testicular carcinoma tissue.

  • General notes

    ab269690 is the PBS only version of ab269680.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)

    Formalin/PFA-fixed paraffin-embedded human testicular carcinoma tissue stained for Vinculin using ab269680 at 2 µg/ml in immunohistochemistry.

    This image was produced using the same antibody clone but in a different formulation containing PBS, BSA and Sodium Azide (ab269680).

  • Western blot - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)
    Western blot - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)
    All lanes : Anti-Vinculin antibody [VCL/2575] (ab269680) at 2 µg/ml

    Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
    Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

    Predicted band size: 124 kDa



    This image was produced using the same antibody clone but in a different formulation containing PBS, BSA and Sodium Azide (ab269680).

  • Protein Array - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)
    Protein Array - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)

    Analysis of Protein Array containing more than 19,000 full-length human proteins using ab269680.
    - and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

    This image was produced using the same antibody clone but in a different formulation containing PBS, BSA and Sodium Azide (ab269680).

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