Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8185] to Vinculin - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Vinculin antibody [EPR8185] - BSA and Azide free
    See all Vinculin primary antibodies

  • Description

    Rabbit monoclonal [EPR8185] to Vinculin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: PC3, HeLa, U937, K562, HUVEC, HepG2 human fetal liver and human fetal kidney lysates ICC/IF: HUVEC cells, HEK293 cells IP: HeLa cells

  • General notes

    Ab217171 is the carrier-free version of ab129002. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab217171 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)
    Flow Cytometry - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)

    Flow cytometry analysis of 293 (human embryonic kidney epithelial) cells labeling Vinculin (red) with ab129002 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129002).

  • Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)
    Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)

    Immunofluorescent staining of HEK293 cells (fixed in 4% PFA, permeabilized with 0.1% Triton X 100) using purified ab129002 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) was used as the secondary at a dilution of 1/1000 and the cells were counter stained with DAPI. The negative controls are shown in the bottom middle and right hand panels. For negative control 1, the primary was used and then goat anti-mouse IgG was used at a dilution of 1/500. For negative control 2, a mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) were used.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129002).

  • Immunoprecipitation - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)
    Immunoprecipitation - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)

    ab129002 (purified) at 1/20 immunoprecipitating vinculin in HeLa cells. Lane 1: HeLa whole cell lysate (10 µg). Lane 2: HeLa whole cell lysate (10 µg). Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab129002 in HeLa whole cell lysate. For western blotting, a HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129002).

  • Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)
    Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)

    Immunofluorescent staining of vinculin in HUVEC cells with unpurified ab129002 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129002).

  • Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)
    Anti-Vinculin antibody [EPR8185] - BSA and Azide free (ab217171)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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