Anti-Ezrin (phospho T567) antibody (ab47293)
Key features and details
- Rabbit polyclonal to Ezrin (phospho T567)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Ezrin (phospho T567) antibody
See all Ezrin primary antibodies -
Description
Rabbit polyclonal to Ezrin (phospho T567) -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of Ezrin only when phosphorylated at Threonine 567. This antibody will also react with Moesin at Threonine 577 and Radixin at Threonine 564. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Ssynthesized phosphopeptide derived from human Ezrin around the phosphorylation site of threonine 567 (Y-K-TP-L-R)
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Positive control
- Extracts from A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was purified using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ezrin (phospho T567) antibody (ab47293)All lanes : Anti-Ezrin (phospho T567) antibody (ab47293)
Lane 1 : EGF treated A431 cells, no added peptide
Lane 2 : Untreated A431 cells, no added peptide
Predicted band size: 69 kDa
Observed band size: 69 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (phospho T567) antibody (ab47293)Ab47293 staining human normal placenta. Staining is localised to apical membrane and cytoplasmic compartiment.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may b -
Western blot - Anti-Ezrin (phospho T567) antibody (ab47293)All lanes : Anti-Ezrin (phospho T567) antibody (ab47293)
Lane 1 : Jurkat cell lysate with PMA (125 ng/ml, 30 minutes)
Lane 2 : Jurkat cell lysate
Predicted band size: 69 kDa
Observed band size: 69 kDa
Additional bands at: 68 kDa (possible cross reactivity)
The lower band is likely to be Moesin, which has a homologous immunogenic region.
