Anti-Vinculin antibody [VCL/2575] (ab269680)
![Anti-Vinculin antibody [VCL/2575] (ab269680)](https://www.abcam.com/ps/products/269/ab269680/Images/ab269680-368412-anti-vinculin-antibody-ihc-human-testicular-carcinoma.jpg "Anti-Vinculin antibody [VCL/2575] (ab269680)")
Key features and details
- Mouse monoclonal [VCL/2575] to Vinculin
- Suitable for: IHC-P, WB, Protein Array
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Vinculin antibody [VCL/2575]
See all Vinculin primary antibodies -
Description
Mouse monoclonal [VCL/2575] to Vinculin -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, Protein Arraymore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment aa 174-322. The exact sequence is proprietary.
Database link: P18206 -
Positive control
- WB: U-87 and THP-1 cell lysates. IHC-P: Human testicular carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from Bioreactor Concentrate -
Clonality
Monoclonal -
Clone number
VCL/2575 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [VCL/2575] (ab269680)
Formalin-fixed, paraffin-embedded human testicular carcinoma tissue stained for Vinculin using ab269680 at 2 μg/ml in immunohistochemical analysis.
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![Western blot - Anti-Vinculin antibody [VCL/2575] (ab269680)](https://www.abcam.com/ps/products/269/ab269680/Images/ab269680-368413-anti-vinculin-antibody-western-blot-human-u87-and-thp1-cell-lysates.jpg)
Western blot - Anti-Vinculin antibody [VCL/2575] (ab269680)All lanes : Anti-Vinculin antibody [VCL/2575] (ab269680) at 2 µg/ml
Lane 1 : U-87 (human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 2 : THP-1 (human monocytic leukemia cell line) cell lysate
Predicted band size: 124 kDa
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![Protein Array - Anti-Vinculin antibody [VCL/2575] (ab269680)](https://www.abcam.com/ps/products/269/ab269680/Images/ab269680-368414-anti-vinculin-antibody-protein-array.jpg)
Protein Array - Anti-Vinculin antibody [VCL/2575] (ab269680)Protein Array containing more than 19,000 full-length human proteins using ab269680.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
