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Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14828] to Syntrophin alpha 1
  • Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Syntrophin alpha 1 antibody [EPR14828]
    See all Syntrophin alpha 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14828] to Syntrophin alpha 1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HT29, HepG2, A549, MCF7, Human muscle and NIH 3T3 lysates; Human brain tissue and bladder transitional cell carcinoma tissue; MCF7 and HT29 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14828

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)
    Western blot - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)
    All lanes : Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873) at 1/1000 dilution

    Lane 1 : HT29 lysate
    Lane 2 : HepG2 lysate
    Lane 3 : A549 lysate
    Lane 4 : MCF7 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Western blot - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)
    Western blot - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)
    Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873) at 1/1000 dilution + Human muscle lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Western blot - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)
    Western blot - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)
    Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873) at 1/1000 dilution + NIH 3T3 lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Syntrophin alpha 1 with ab188873 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)

    Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling Syntrophin alpha 1 with ab188873 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)
    Immunocytochemistry/ Immunofluorescence - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling Syntrophin alpha 1 with ab188873 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).

  • Flow Cytometry - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)
    Flow Cytometry - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HT29 cells labeling Syntrophin alpha 1 with ab188873 at 1/280 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)
    Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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