Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14828] to Syntrophin alpha 1
Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
Reacts with: Mouse, Human

Product name

Anti-Syntrophin alpha 1 antibody [EPR14828]
See all Syntrophin alpha 1 primary antibodies
Description

Rabbit monoclonal [EPR14828] to Syntrophin alpha 1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HT29, HepG2, A549, MCF7, Human muscle and NIH 3T3 lysates; Human brain tissue and bladder transitional cell carcinoma tissue; MCF7 and HT29 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14828
Isotype

IgG
Research areas

Western blot - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)

All lanes : Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873) at 1/1000 dilution

Lane 1 : HT29 lysate
Lane 2 : HepG2 lysate
Lane 3 : A549 lysate
Lane 4 : MCF7 lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 54 kDa
Observed band size: 54 kDa
Western blot - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)

Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873) at 1/1000 dilution + Human muscle lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 54 kDa
Observed band size: 54 kDa
Western blot - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)

Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873) at 1/1000 dilution + NIH 3T3 lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 54 kDa
Observed band size: 54 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Syntrophin alpha 1 with ab188873 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)

Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling Syntrophin alpha 1 with ab188873 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling Syntrophin alpha 1 with ab188873 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).
Flow Cytometry - Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)

Flow cytometric analysis of 2% paraformaldehyde-fixed HT29 cells labeling Syntrophin alpha 1 with ab188873 at 1/280 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Anti-Syntrophin alpha 1 antibody [EPR14828] (ab188873)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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