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Anti-Syntrophin alpha 1 antibody [EPR14828] - BSA and Azide free (ab239008)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14828] to Syntrophin alpha 1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Syntrophin alpha 1 antibody [EPR14828] - BSA and Azide free
    See all Syntrophin alpha 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14828] to Syntrophin alpha 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human brain tissue.

  • General notes

    Ab239008 is the carrier-free version of ab188873. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239008 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-Syntrophin alpha 1 antibody [EPR14828] - BSA and Azide free (ab239008)
    Flow Cytometry - Anti-Syntrophin alpha 1 antibody [EPR14828] - BSA and Azide free (ab239008)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HT29 cells labeling Syntrophin alpha 1 with ab188873 at 1/280 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188873).

  • Immunocytochemistry/ Immunofluorescence - Anti-Syntrophin alpha 1 antibody [EPR14828] - BSA and Azide free (ab239008)
    Immunocytochemistry/ Immunofluorescence - Anti-Syntrophin alpha 1 antibody [EPR14828] - BSA and Azide free (ab239008)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling Syntrophin alpha 1 with ab188873 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188873).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntrophin alpha 1 antibody [EPR14828] - BSA and Azide free (ab239008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntrophin alpha 1 antibody [EPR14828] - BSA and Azide free (ab239008)

    Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling Syntrophin alpha 1 with ab188873 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188873).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntrophin alpha 1 antibody [EPR14828] - BSA and Azide free (ab239008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syntrophin alpha 1 antibody [EPR14828] - BSA and Azide free (ab239008)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Syntrophin alpha 1 with ab188873 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188873).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Syntrophin alpha 1 antibody [EPR14828] - BSA and Azide free (ab239008)
    Anti-Syntrophin alpha 1 antibody [EPR14828] - BSA and Azide free (ab239008)

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