Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PMP70 antibody [EPR5614] (ab109448)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5614] to PMP70
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PMP70 antibody [EPR5614]
    See all PMP70 primary antibodies

  • Description

    Rabbit monoclonal [EPR5614] to PMP70

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Hap1, HeLa, 293T, SH SY5Y, Caco2, and HepG2 cell lysates. Flow Cyt: Permeabilized HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PMP70 antibody [EPR5614] (ab109448)
    Western blot - Anti-PMP70 antibody [EPR5614] (ab109448)
    All lanes : Anti-PMP70 antibody [EPR5614] (ab109448) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : ABCD3 knockout HeLa cell lysate

    Lysates/proteins at 40 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 75 kDa
    Observed band size: 75 kDa



    Lanes 1- 2: Merged signal (red and green). Green - ab109448 observed at 75 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab109448 was shown to react with PMP70 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265294 (knockout cell lysate ab257134) was used. Wild-type HeLa and ABCD3 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109448 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-PMP70 antibody [EPR5614] (ab109448)
    Flow Cytometry - Anti-PMP70 antibody [EPR5614] (ab109448)
    ab109448 at 1/100 dilution staining PMP70 in permeabilized HepG2 cells by Flow cytometry (shown in red). Rabbit IgG negative control (shown in green).
  • Western blot - Anti-PMP70 antibody [EPR5614] (ab109448)
    Western blot - Anti-PMP70 antibody [EPR5614] (ab109448)
    All lanes : Anti-PMP70 antibody [EPR5614] (ab109448) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : ABCD3 (PMP70) knockout HAP1 whole cell lysate
    Lane 3 : A431 whole cell lysate
    Lane 4 : HEPG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 75 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab109448 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab109448 was shown to specifically react with PMP70 in wild-type HAP1 cells as signal was lost in ABCD3 (PMP70) knockout cells. Wild-type and ABCD3 (PMP70) knockout samples were subjected to SDS-PAGE. Ab109448 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PMP70 antibody [EPR5614] (ab109448)
    Western blot - Anti-PMP70 antibody [EPR5614] (ab109448)
    All lanes : Anti-PMP70 antibody [EPR5614] (ab109448) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : SH SY5Y cell lysate
    Lane 3 : Caco2 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 75 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-PMP70 antibody [EPR5614] (ab109448)
    OI-RD Scanning - Anti-PMP70 antibody [EPR5614] (ab109448)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PMP70 antibody [EPR5614] (ab109448)
    Anti-PMP70 antibody [EPR5614] (ab109448)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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