Biotin Anti-PMP70 antibody (ab28499)
Key features and details
- Biotin Rabbit polyclonal to PMP70
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-PMP70 antibody
See all PMP70 primary antibodies -
Description
Biotin Rabbit polyclonal to PMP70 -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
ab28499 recognises Peroxisomal membrane protein 70 (PMP70). -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken
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Immunogen
Synthetic peptide:
NYEFKKITEDTVEFGS
, corresponding to C terminal amino acids 644 - 659 of Rat PMP70 -
Positive control
- Mouse kidney protein extract
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Biotin Anti-PMP70 antibody (ab28499)All lanes : Biotin Anti-PMP70 antibody (ab28499) at 1/1000 dilution
Lane 1 : Mouse lung tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit
Predicted band size: 74 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Biotin Anti-PMP70 antibody (ab28499)Immunofluorescent analysis of PMP70 staining in A459 cells with ab28499.
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Immunocytochemistry/ Immunofluorescence - Biotin Anti-PMP70 antibody (ab28499)Immunofluorescent analysis of PMP70 staining in NS-1 cells with ab28499.
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Immunocytochemistry/ Immunofluorescence - Biotin Anti-PMP70 antibody (ab28499)Immunofluorescent analysis of PMP70 staining in HMVEC cells with ab28499.
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Immunocytochemistry/ Immunofluorescence - Biotin Anti-PMP70 antibody (ab28499)Immunofluorescent analysis of PMP70 staining in p19 cells with ab28499.
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Flow Cytometry - Biotin Anti-PMP70 antibody (ab28499)Flow cytometry analysis of PMP70 in HepG2 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x106 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab28499 at a dilution of 0.5 μg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight® 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
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Flow Cytometry - Biotin Anti-PMP70 antibody (ab28499)Flow cytometry analysis of PMP70 in 293T cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x106 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab28499 at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight® 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
