Anti-Fibronectin antibody [M013] (ab253287)
![Anti-Fibronectin antibody [M013] (ab253287)](https://www.abcam.com/ps/products/253/ab253287/Images/ab253287-344296-AntiFibronectin-antibody-M013-immunocytochemistry-a549-human.jpg "Anti-Fibronectin antibody [M013] (ab253287)")
Key features and details
- Mouse monoclonal [M013] to Fibronectin
- Suitable for: WB, ICC/IF, ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Fibronectin antibody [M013]
See all Fibronectin primary antibodies -
Description
Mouse monoclonal [M013] to Fibronectin -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Full length native protein (purified) within Human Fibronectin. The exact sequence is proprietary. From the A549 lung carcinoma cell line.
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Positive control
- WB: A549, MeWo and MDA-MB-231 cell lysate. Human plasma fibronectin. ICC/IF: A549 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
M013 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [M013] (ab253287)
Paraformaldehyde-fixed, A549 (Human lung carcinoma cell line) cells labeling Fibronectin using ab253287 at a 1/50 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.
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![Western blot - Anti-Fibronectin antibody [M013] (ab253287)](https://www.abcam.com/ps/products/253/ab253287/Images/ab253287-344295-AntiFibronectin-antibody-M013-western-blot-a549-mewo-mdamb231-plasma-human.jpg)
Western blot - Anti-Fibronectin antibody [M013] (ab253287)All lanes : Anti-Fibronectin antibody [M013] (ab253287) at 1/1000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) cell lysate
Lane 2 : MeWo (Human malignant melanoma cell line) cell lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) cell lysate
Lane 4 : Human plasma fibronectinNative conditions.
Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hr at room temperature.
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![ELISA - Anti-Fibronectin antibody [M013] (ab253287)](https://www.abcam.com/ps/products/253/ab253287/Images/ab253287-382412-anti-fibronectin-antibody-m013-elisa.jpg)
ELISA - Anti-Fibronectin antibody [M013] (ab253287)Representative Standard Curve using ab253287 for ELISA capture of human recombinant fibronectin protein. Capture was detected by using anti-fibronectin ab253287 followed by streptavidin conjugated to HRP.