Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-RICTOR antibody [EPR22008] (ab219950)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22008] to RICTOR
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-RICTOR antibody [EPR22008]
See all RICTOR primary antibodies
Description

Rabbit monoclonal [EPR22008] to RICTOR
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HAP1, HeLa, HEK-293, HCT 116, MDA-MB-231, SW480 and PC-3 whole cell lysate. Flow Cyt: HCT 116 and HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22008
Isotype

IgG
Research areas

Western blot - Anti-RICTOR antibody [EPR22008] (ab219950)

All lanes : Anti-RICTOR antibody [EPR22008] (ab219950) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate at 20 µg
Lane 2 : RICTOR knockout HAP1 whole cell lysate at 20 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 192 kDa

Blocking/Diluting buffer and concentration: 5%NFDM/TBST.

Exposure time: 26 seconds.

ab219950 was shown to specifically react with RICTOR in wild-type HAP1 cells as signal was lost in RICTOR knockout cells. Wild-type and RICTOR knockout samples were subjected to SDS-PAGE. ab219950 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/50,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument using the ECL technique.
Western blot - Anti-RICTOR antibody [EPR22008] (ab219950)

All lanes : Anti-RICTOR antibody [EPR22008] (ab219950) at 1/1000 dilution

Lane 1 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate
Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : SW480 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 4 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 2-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 192 kDa

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

Exposure times: Lane 1: 3 minutes; Lane 2: 48 seconds; Lane 3-4: 26 seconds.
Flow Cytometry - Anti-RICTOR antibody [EPR22008] (ab219950)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling RICTOR with ab219950 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry - Anti-RICTOR antibody [EPR22008] (ab219950)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HCT 116 (human colorectal carcinoma cell line) cell line labeling RICTOR with ab219950 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
Anti-RICTOR antibody [EPR22008] (ab219950)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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