Anti-RICTOR antibody (ab245371)
Key features and details
- Rabbit polyclonal to RICTOR
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RICTOR antibody
See all RICTOR primary antibodies -
Description
Rabbit polyclonal to RICTOR -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP Human -
Immunogen
Synthetic peptide within Human RICTOR aa 1-50. The exact sequence is proprietary. NP_689969.2
Database link: Q6R327 -
Positive control
- IP: HEK-293T whole cell lysate. IHC-P: Human prostate carcinoma tissue.
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General notes
ab245371 has not performed satisfactorily when used for WB of RICTOR in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RICTOR.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab245371 was affinity purified using an epitope specific to RICTOR immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for RICTOR using ab245371 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
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RICTOR was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245371 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using a different Anti-RICTOR antibody at 1 µg/ml.
Lane 1: ab245371 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.