Anti-RICTOR antibody [1G3P2C9] (ab176850)
Key features and details
- Mouse monoclonal [1G3P2C9] to RICTOR
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-RICTOR antibody [1G3P2C9]
See all RICTOR primary antibodies -
Description
Mouse monoclonal [1G3P2C9] to RICTOR -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human RICTOR aa 1650-1708 (C terminal). The exact sequence is proprietary.
Sequence:I CLYSEVSHLL SHCTFRLPCR RFIQELFQDV QFLQMHEEAE AVLATPPKQP IVDTSAES
Database link: Q6R327 -
Epitope
The epitope recognized by ab176850 maps to a region between residue 1650 and the C-terminus (residue 1708) using the numbering given in Q6R327. -
Positive control
- Human prostate carcinoma tissue; 293T whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab176850 was affinity purified using an epitope specific to RICTOR immobilized on solid support. -
Clonality
Monoclonal -
Clone number
1G3P2C9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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All lanes : Anti-RICTOR antibody [1G3P2C9] (ab176850) at 1 µg/ml
Lane 1 : 293T whole cell lysates at 50 µg
Lane 2 : 293T whole cell lysates at 15 µg
Lane 3 : 293T whole cell lysates at 5 µg
Developed using the ECL technique.
Predicted band size: 192 kDa
Exposure time: 10 seconds
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling RICTOR with ab176850 at 1/250 dilution. Detection: DAB staining.