Anti-RICTOR antibody (ab70374)
Key features and details
- Rabbit polyclonal to RICTOR
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RICTOR antibody
See all RICTOR primary antibodies -
Description
Rabbit polyclonal to RICTOR -
Host species
Rabbit -
Specificity
Initial tests for reactivity with mouse RICTOR from NIH3T3 cells suggest that higher amounts of lysate and less dilution of ab70374 are required for brief exposure times. -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to a region between residue 1650 and the C-terminus (residue 1708) of human RICTOR.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RICTOR antibody (ab70374)All lanes : Anti-RICTOR antibody (ab70374) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 5 µg
Lane 2 : HeLa whole cell lysate at 2.5 µg
Lane 3 : HeLa whole cell lysate at 1 µg
Lane 4 : HeLa whole cell lysate at 0.5 µg
Lane 5 : NIH3T3 cells at 50 µg
Predicted band size: 192 kDa
Observed band size: 192 kDa
Additional bands at: 78 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody (ab70374)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling RICTOR with ab70374 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-RICTOR antibody (ab70374)Detection of Human RICTOR by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded) using ab70374 at 3 µg/mg for IP (Lane 1) and at 0.04 µg/ml for subsequent WB detection. Lane 2 represents control rabbit IgG. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RICTOR antibody (ab70374)ab70374 (2µg/ml) staining RICTOR in human prostate using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of glandular epithelium.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
