Anti-MFF antibody (ab242102)
Key features and details
- Rabbit polyclonal to MFF
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MFF antibody
See all MFF primary antibodies -
Description
Rabbit polyclonal to MFF -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Orangutan
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Immunogen
Synthetic peptide within Human MFF aa 50-100. The exact sequence is proprietary.
Database link: Q9GZY8 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to MFF immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MFF antibody (ab242102)All lanes : Anti-MFF antibody (ab242102) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 2 : HeLa whole cell lysate at 5 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 38 kDa
Exposure time: 30 secondsLysates prepared in NETN buffer.
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Immunoprecipitation - Anti-MFF antibody (ab242102)MFF was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab242102 at 20 µl/reaction. Western blot was performed from the immunoprecipitate using ab242102 at 1/250 dilution.
Lane 1: ab242102 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
