Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1965] to Cip4 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cip4 antibody [EPR1965] - BSA and Azide free
    See all Cip4 primary antibodies

  • Description

    Rabbit monoclonal [EPR1965] to Cip4 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Recent WB re-tests performed by our lab suggest to use a higher dilution for mouse samples. The tested dilutions were 1:500 and 1:2000, were the signal detected was very strong. Human and rat samples worked fine in these dilutions.

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, JAR, TF1, HeLa and HepG2 cell lysates. IHC-P: Human stomach tissue.

  • General notes

    Ab247627 is the carrier-free version of ab108313. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247627 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
    Western blot - Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
    All lanes : Anti-Cip4 antibody [EPR1965] (ab108313) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : TRIP10 knockout HEK293T cell lysate
    Lane 3 : JAR cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 68 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab108313, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab108313 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab108313 Anti-Cip4 antibody [EPR1965] was shown to specifically react with Cip4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266428 (knockout cell lysate ab258251) was used. Wild-type and Cip4 knockout samples were subjected to SDS-PAGE. ab108313 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
    This data was developed using ab108313, the same antibody clone in a different buffer formulation.ab108313 at 1/100 dilution staining Cip4 in paraffin embedded Human stomach tissue. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Western blot - Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
    Western blot - Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
    All lanes : Anti-Cip4 antibody [EPR1965] (ab108313) at 1/1000 dilution

    Lane 1 : JAR cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : TF1 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 68 kDa



    This data was developed using ab108313, the same antibody clone in a different buffer formulation.

  • Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)
    Anti-Cip4 antibody [EPR1965] - BSA and Azide free (ab247627)

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