Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8975] to Metabotropic Glutamate Receptor 2/MGLUR2
- Suitable for: IHC-P, WB, Flow Cyt, ICC
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975]
See all Metabotropic Glutamate Receptor 2/MGLUR2 primary antibodies -
Description
Rabbit monoclonal [EPR8975] to Metabotropic Glutamate Receptor 2/MGLUR2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC MouseIHC-P HumanWB MouseRatHuman -
Immunogen
Synthetic peptide corresponding to Human Metabotropic Glutamate Receptor 2/MGLUR2 aa 850 to the C-terminus (C terminal). The immunogen used for this product shares 92% homology with Metabotropic glutamate receptor 3. Cross-reactivity with this protein has not been confirmed experimentally.
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Positive control
- WB: Human hypothalamus, cerebellum and fetal forebrain cell lysates; Rat and mouse brain lysates. IHC-P: Human fetal brain tissue. Flow Cyt: SH-SY-SY cells. ICC: Mouse primary neurone cells
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General notes
This product was previously labelled as Metabotropic Glutamate Receptor 2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8975 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry analysis of mouse primary neurone cells labelling MGLUR2 with ab150387 at 2 µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-MAP2 rabbit monoclonal antibody (ab11267) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue).
Confocal image showing positive staining in mouse primary neuron cells.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
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All lanes : Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 95 kDa
Observed band size: 95 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 180 s
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Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Metabotropic Glutamate Receptor 2/MGLUR2 with unpurified ab150387 at 1/70 dilution (10µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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Immunohistochemical analysis of Human fetal brain tissue labelling Metabotropic Glutamate Receptor 2/MGLUR2 with ab150387 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387) at 1/10000 dilution
Lane 1 : Human hypothalamus cell lysate
Lane 2 : Human fetal brain cell lysate
Lane 3 : Human cerebellum cell lysate
Lane 4 : Human fetal forebrain cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 95 kDa
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Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150387 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab150387 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab150387 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab150387 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Fetal kidney tissue using ab150387 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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