Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8975] to Metabotropic Glutamate Receptor 2/MGLUR2
Suitable for: IHC-P, WB, Flow Cyt, ICC
Reacts with: Mouse, Rat, Human

Product name

Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975]
See all Metabotropic Glutamate Receptor 2/MGLUR2 primary antibodies
Description

Rabbit monoclonal [EPR8975] to Metabotropic Glutamate Receptor 2/MGLUR2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC	Mouse
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Metabotropic Glutamate Receptor 2/MGLUR2 aa 850 to the C-terminus (C terminal). The immunogen used for this product shares 92% homology with Metabotropic glutamate receptor 3. Cross-reactivity with this protein has not been confirmed experimentally.
Positive control
- WB: Human hypothalamus, cerebellum and fetal forebrain cell lysates; Rat and mouse brain lysates. IHC-P: Human fetal brain tissue. Flow Cyt: SH-SY-SY cells. ICC: Mouse primary neurone cells
General notes
This product was previously labelled as Metabotropic Glutamate Receptor 2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8975
Isotype

IgG
Research areas

Immunocytochemistry - Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)

Immunocytochemistry analysis of mouse primary neurone cells labelling MGLUR2 with ab150387 at 2 µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-MAP2 rabbit monoclonal antibody (ab11267) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue).

Confocal image showing positive staining in mouse primary neuron cells.

Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
Western blot - Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)

All lanes : Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 95 kDa
Observed band size: 95 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 180 s
Flow Cytometry - Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)

Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Metabotropic Glutamate Receptor 2/MGLUR2 with unpurified ab150387 at 1/70 dilution (10µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)

Immunohistochemical analysis of Human fetal brain tissue labelling Metabotropic Glutamate Receptor 2/MGLUR2 with ab150387 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)

All lanes : Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387) at 1/10000 dilution

Lane 1 : Human hypothalamus cell lysate
Lane 2 : Human fetal brain cell lysate
Lane 3 : Human cerebellum cell lysate
Lane 4 : Human fetal forebrain cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-labelled goat anti-rabbit antibody at 1/2000 dilution

Predicted band size: 95 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150387 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)

Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab150387 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab150387 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)

Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab150387 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)

Immunohistochemical analysis of paraffin embedded Human Fetal kidney tissue using ab150387 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Metabotropic Glutamate Receptor 2/MGLUR2 antibody [EPR8975] (ab150387)

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