Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CAD/BM1 antibody [EP710Y] (ab40800)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP710Y] to CAD/BM1
Suitable for: ICC/IF, WB, IP, Flow Cyt
Reacts with: Human

Product name

Anti-CAD/BM1 antibody [EP710Y]
See all CAD/BM1 primary antibodies
Description

Rabbit monoclonal [EP710Y] to CAD/BM1
Host species

Rabbit
Specificity

ab40800 is specific to the N-terminus of CAD/BM1.
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human CAD/BM1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P27708
Positive control
- WB: HeLa and HEK293 cell lysate. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate (ab150035).
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product was previously labelled as CAD
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP710Y
Isotype

IgG
Research areas
- Cell Biology
- Apoptosis
- Nucleus
- DNAses
- Cancer
- Cell Death
- Apoptosis
- Nucleus
- DNAses

Western blot - Anti-CAD/BM1 antibody [EP710Y] (ab40800)

Anti-CAD/BM1 antibody [EP710Y] (ab40800) at 1/2000 dilution (purified) + HeLa cell lysate at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Predicted band size: 243 kDa
Observed band size: 250 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-CAD/BM1 antibody [EP710Y] (ab40800)

Immunofluorescence staining of HeLa cells with purified ab40800 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab40800 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Flow Cytometry - Anti-CAD/BM1 antibody [EP710Y] (ab40800)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CAD/BM1 (red) with ab40800 at a 1/120 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Immunoprecipitation - Anti-CAD/BM1 antibody [EP710Y] (ab40800)

ab40800 (purified) at 1/30 immunoprecipitating CAD/BM1 in 10 μg HeLa (Lanes 1 and 2, observed at 250 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP (ab131366) was used for detection (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
Western blot - Anti-CAD/BM1 antibody [EP710Y] (ab40800)

Anti-CAD/BM1 antibody [EP710Y] (ab40800) at 1/1000 dilution (purified) + HeLa cell lysate at 1/20000 dilution

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Predicted band size: 243 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-CAD/BM1 antibody [EP710Y] (ab40800)

Unpurified ab40800 (1/100), staining human CAD/BM1 (N-term) in HeLa cells by Immunofluorescence.
Western blot - Anti-CAD/BM1 antibody [EP710Y] (ab40800)

Anti-CAD/BM1 antibody [EP710Y] (ab40800) at 1/1000 dilution (unpurified) + HEK-293 whole cell lysate (ab7902) at 10 µg

Predicted band size: 243 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Anti-CAD/BM1 antibody [EP710Y] (ab40800)

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