Anti-CAD/BM1 antibody [EP711Y] (ab40765)
![Anti-CAD/BM1 antibody [EP711Y] (ab40765)](https://www.abcam.com/ps/products/40/ab40765/Images/ab40765-415223-anti-cad-bm1-antibody-ep711y-westernblot-hek-293-human.jpg "Anti-CAD/BM1 antibody [EP711Y] (ab40765)")
Key features and details
- Rabbit monoclonal [EP711Y] to CAD/BM1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CAD/BM1 antibody [EP711Y]
See all CAD/BM1 primary antibodies -
Description
Rabbit monoclonal [EP711Y] to CAD/BM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CAD/BM1 aa 2150-2250 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HEK-293 cell lysate IHC-P: Human breast carcinoma
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as CAD
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP711Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CAD/BM1 antibody [EP711Y] (ab40765)Anti-CAD/BM1 antibody [EP711Y] (ab40765) at 1/2000 dilution (Unpurified) + HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 250 kDa
Observed band size: 245 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAD/BM1 antibody [EP711Y] (ab40765)](https://www.abcam.com/ps/products/40/ab40765/Images/ab40765-53890-anti-cad-antibody-ep711y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAD/BM1 antibody [EP711Y] (ab40765)Immunohistochemical analysis of paraffin-embedded human breast carcinoma
using ab40765 at 1/250 dilution.Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
