Antibodies, Proteins, Kits and Reagents for Life Science

Anti-VEGF Receptor 1 antibody [EWI] (ab238632)

Key features and details

Mouse monoclonal [EWI] to VEGF Receptor 1
Suitable for: Flow Cyt, WB
Reacts with: Human, Pig, Spodoptera frugiperda (SF9 cells)
Isotype: IgG1

Product name

Anti-VEGF Receptor 1 antibody [EWI]
See all VEGF Receptor 1 primary antibodies
Description

Mouse monoclonal [EWI] to VEGF Receptor 1
Host species

Mouse
Specificity

This antibody can detect the native form of VEGF Receptor 1.
Tested applications

Suitable for: Flow Cyt, WBmore details
Species reactivity

Reacts with: Human, Pig, Spodoptera frugiperda (SF9 cells)
Immunogen

Recombinant full length protein corresponding to Human VEGF Receptor 1 aa 1-600. Soluble form.
Database link: P17948-1

Run BLAST with Run BLAST with
Positive control
- WB: Recombinant human VEGF Receptor 1; Conditioned supernatant of insect cells expressing VEGF Receptor 1. Recombinant human soluble VEGFR 1 (sFlt-1 (D1-D7)-hFc), (sFlt-1 (native)) and (sFlt-1 (D1-D3)) proteins. Flow Cyt: Porcine aortic endothelial cells (PAE/Flt1) expressing human VEGF Receptor 1.

Form

Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

EWI
Isotype

IgG1
Research areas

Western blot - Anti-VEGF Receptor 1 antibody [EWI] (ab238632)

All lanes : Anti-VEGF Receptor 1 antibody [EWI] (ab238632) at 10 µg/ml

Lane 1 : Recombinant human soluble VEGF Receptor 1 derived from insect cells
Lane 2 : Supernatant from Adenoviris infected A549 cells expressing mouse VEGF Receptor 1

Predicted band size: 151 kDa
Flow Cytometry - Anti-VEGF Receptor 1 antibody [EWI] (ab238632)

Flow cytometric analysis of porcine aortic endothelial cells expressing human VEGF Receptor 1 using ab238632 at 10 μg/ml.
Western blot - Anti-VEGF Receptor 1 antibody [EWI] (ab238632)

All lanes : Anti-VEGF Receptor 1 antibody [EWI] (ab238632) at 10 µg/ml

Lane 1 : Recombinant human soluble VEGFR 1 (sFlt-1 (D1-D7)-hFc)
Lane 2 : Recombinant human soluble VEGFR 1 (sFlt-1 (native))
Lane 3 : Recombinant human soluble VEGFR 1 (sFlt-1 (D1-D3))

Predicted band size: 151 kDa
Western blot - Anti-VEGF Receptor 1 antibody [EWI] (ab238632)

All lanes : Anti-VEGF Receptor 1 antibody [EWI] (ab238632) at 10 µg/ml

All lanes : Recombinant human endogenous soluble VEGF Receptor 1 with sf9 derived conditioned supernatant (Insect cells).

Predicted band size: 151 kDa

10% SDS-PAGE
Western blot - Anti-VEGF Receptor 1 antibody [EWI] (ab238632)

All lanes : Anti-VEGF Receptor 1 antibody [EWI] (ab238632) at 10 µg/ml

Lane 1 : Soluble recombinant VEGF Receptor 1 at 2 µg
Lane 2 : Endogenous soluble recombinant VEGF Receptor 1 at 1 µg
Lane 3 : Soluble recombinant VEGF Receptor 2 at 2 µg

Predicted band size: 151 kDa

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