Anti-VEGF Receptor 1 antibody [EWI] (ab238632)
![Anti-VEGF Receptor 1 antibody [EWI] (ab238632)](https://www.abcam.com/ps/products/238/ab238632/Images/ab238632-316617-anti-vegf-receptor-1-antibody-ewi-western-blot.jpg "Anti-VEGF Receptor 1 antibody [EWI] (ab238632)")
Key features and details
- Mouse monoclonal [EWI] to VEGF Receptor 1
- Suitable for: Flow Cyt, WB
- Reacts with: Human, Pig, Spodoptera frugiperda (SF9 cells)
- Isotype: IgG1
Overview
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Product name
Anti-VEGF Receptor 1 antibody [EWI]
See all VEGF Receptor 1 primary antibodies -
Description
Mouse monoclonal [EWI] to VEGF Receptor 1 -
Host species
Mouse -
Specificity
This antibody can detect the native form of VEGF Receptor 1. -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human, Pig, Spodoptera frugiperda (SF9 cells) -
Immunogen
Recombinant full length protein corresponding to Human VEGF Receptor 1 aa 1-600. Soluble form.
Database link: P17948-1 -
Positive control
- WB: Recombinant human VEGF Receptor 1; Conditioned supernatant of insect cells expressing VEGF Receptor 1. Recombinant human soluble VEGFR 1 (sFlt-1 (D1-D7)-hFc), (sFlt-1 (native)) and (sFlt-1 (D1-D3)) proteins. Flow Cyt: Porcine aortic endothelial cells (PAE/Flt1) expressing human VEGF Receptor 1.
Properties
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Form
Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
EWI -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-VEGF Receptor 1 antibody [EWI] (ab238632)All lanes : Anti-VEGF Receptor 1 antibody [EWI] (ab238632) at 10 µg/ml
Lane 1 : Recombinant human soluble VEGF Receptor 1 derived from insect cells
Lane 2 : Supernatant from Adenoviris infected A549 cells expressing mouse VEGF Receptor 1
Predicted band size: 151 kDa
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![Flow Cytometry - Anti-VEGF Receptor 1 antibody [EWI] (ab238632)](https://www.abcam.com/ps/products/238/ab238632/Images/ab238632-316615-anti-vegf-receptor-1-antibody-ewi-flow-cytometry.jpg)
Flow Cytometry - Anti-VEGF Receptor 1 antibody [EWI] (ab238632)Flow cytometric analysis of porcine aortic endothelial cells expressing human VEGF Receptor 1 using ab238632 at 10 μg/ml.
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![Western blot - Anti-VEGF Receptor 1 antibody [EWI] (ab238632)](https://www.abcam.com/ps/products/238/ab238632/Images/ab238632-316614-anti-vegf-receptor-1-antibody-ewi-western-blot.jpg)
Western blot - Anti-VEGF Receptor 1 antibody [EWI] (ab238632)All lanes : Anti-VEGF Receptor 1 antibody [EWI] (ab238632) at 10 µg/ml
Lane 1 : Recombinant human soluble VEGFR 1 (sFlt-1 (D1-D7)-hFc)
Lane 2 : Recombinant human soluble VEGFR 1 (sFlt-1 (native))
Lane 3 : Recombinant human soluble VEGFR 1 (sFlt-1 (D1-D3))
Predicted band size: 151 kDa
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![Western blot - Anti-VEGF Receptor 1 antibody [EWI] (ab238632)](https://www.abcam.com/ps/products/238/ab238632/Images/ab238632-316612-anti-vegf-receptor-1-antibody-ewi-western-blot.jpg)
Western blot - Anti-VEGF Receptor 1 antibody [EWI] (ab238632)All lanes : Anti-VEGF Receptor 1 antibody [EWI] (ab238632) at 10 µg/ml
All lanes : Recombinant human endogenous soluble VEGF Receptor 1 with sf9 derived conditioned supernatant (Insect cells).
Predicted band size: 151 kDa10% SDS-PAGE
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![Western blot - Anti-VEGF Receptor 1 antibody [EWI] (ab238632)](https://www.abcam.com/ps/products/238/ab238632/Images/ab238632-316613-anti-vegf-receptor-1-antibody-ewi-western-blot.jpg)
Western blot - Anti-VEGF Receptor 1 antibody [EWI] (ab238632)All lanes : Anti-VEGF Receptor 1 antibody [EWI] (ab238632) at 10 µg/ml
Lane 1 : Soluble recombinant VEGF Receptor 1 at 2 µg
Lane 2 : Endogenous soluble recombinant VEGF Receptor 1 at 1 µg
Lane 3 : Soluble recombinant VEGF Receptor 2 at 2 µg
Predicted band size: 151 kDa
