Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y103] to VEGF Receptor 1
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-VEGF Receptor 1 antibody [Y103]
    See all VEGF Receptor 1 primary antibodies

  • Description

    Rabbit monoclonal [Y103] to VEGF Receptor 1

  • Host species

    Rabbit

  • Specificity

    Based on the antibody's immunogen sequence, it recognises 151 kDa VEGF receptor 1/Flt1, splice isoforms sFlt1 (77 kDa) and sFlt1-14 (82 kDa), and isoform 4 (61 kDa). The sequence is not present in isoforms 5-8 based on Uniprot ID P17948.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Mouse
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human VEGF Receptor 1 aa 1-100 (N terminal). The exact sequence is proprietary.
    (Peptide available as ab182457)

  • Positive control

    • WB: Human and mouse brain tissue; IHC-P: Human gastric carcinoma tissue; IHC-Fr: Mouse brain tissue; IP: Mouse brain lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)
    Western blot - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)
    All lanes : Anti-VEGF Receptor 1 antibody [Y103] (ab32152) at 1/1000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Human brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 151 kDa
    Observed band size: 180 kDa
    why is the actual band size different from the predicted?


    Exposure time: 40 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling VEGF with ab32152, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human gastric carcinoma. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunoprecipitation - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)
    Immunoprecipitation - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

    VEGF Receptor 1 was immunoprecipitated from 0.35 mg mouse brain lysate 10μg with ab32152 at 1:30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab32152 1:1000 dilution (2 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.

    Lane 1: Mouse brain lysate 10μg.

    Lane 2: ab32152 IP in mouse brain lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab32152 in mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody [Y103] (ab32152) Image from Sano, M et al., PLoS ONE. 2014 Mar 20; 9(3). Fig 2E. DOI 10.1371/journal.pone.0089830. Lymphangiogenesis and Angiogenesis in Abdominal Aortic Aneurysm., e89830.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human abdominal aortic aneurysm (AAA) wall tissue sections labeling VEGF Receptor 1 with ab32152 at 1/100 dilution.

    Resected aortic tissues were immersed in 10% neutral buffered formalin for at least 24 h for immunohistochemical staining. Tissue sample was embedded in paraffin; 4 µm sections were cut and mounted onto MAS-coated slides. The sections were deparaffinized, dehydrated, and boiled in a pressure cooker in 0.01 M citric acid buffer (pH 6.0) for 20 min. The sections were washed with phosphate-buffered saline and incubated with 3% H2O2 in absolute methanol for 5 min to inhibit any endogenous peroxidase activity. Sections were preincubated with 3% normal goat serum for 20 min to minimize nonspecific binding to VEGF Receptor 1, and incubated with ab32152 at 4°C overnight in a moist chamber. The section was washed with phosphate-buffered saline and then incubated with the appropriate secondary antibody for 30 min at room temperature. Staining was visualized with Vector DAB, and tissue section was then counterstained with hematoxylin.

  • Western blot - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)
    Western blot - Anti-VEGF Receptor 1 antibody [Y103] (ab32152)
    Anti-VEGF Receptor 1 antibody [Y103] (ab32152) at 1/10000 dilution + mouse brain tissue

    Predicted band size: 151 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?

  • Anti-VEGF Receptor 1 antibody [Y103] (ab32152)
    Anti-VEGF Receptor 1 antibody [Y103] (ab32152)

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