Anti-eIF3K antibody [2313C2a] (ab50736)
![Anti-eIF3K antibody [2313C2a] (ab50736)](https://www.abcam.com/ps/products/50/ab50736/Images/ab50736-414932-anti-eif3k-antibody-2313c2a-western-blot-human-knockout-lysate.jpg "Anti-eIF3K antibody [2313C2a] (ab50736)")
Key features and details
- Mouse monoclonal [2313C2a] to eIF3K
- Suitable for: WB
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-eIF3K antibody [2313C2a]
See all eIF3K primary antibodies -
Description
Mouse monoclonal [2313C2a] to eIF3K -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB HumanRecombinant fragment
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Immunogen
Recombinant human EIF3S12
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Positive control
- WB: HEK293 and THP1 cell lysates; Recombinant human EIF3S12.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab50736 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
2313C2a -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)All lanes : Anti-eIF3K antibody [2313C2a] (ab50736) at 1/500 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : EIF3K knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : THP1 (Human monocytic leukemia cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDaLanes 1-3: Merged signal (red and green). Green - ab50736 observed at 25 kDa. Red - loading control ab181602 observed at 36 kDa.
ab50736 Anti-eIF3K antibody [2313C2a] was shown to specifically react with eIF3K in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266841 (knockout cell lysate ab258857) was used. Wild-type and eIF3K knockout samples were subjected to SDS-PAGE. ab50736 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)](https://www.abcam.com/ps/products/50/ab50736/Images/ab50736.bmp)
Western blot - Anti-eIF3K antibody [2313C2a] (ab50736)Anti-eIF3K antibody [2313C2a] (ab50736) + immunized recombinant protein
Predicted band size: 25 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
